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Literature summary extracted from

  • Kimura, A.K.; Kim, H.Y.
    Phosphatidylserine synthase 2: high efficiency for synthesizing phosphatidylserine containing docosahexaenoic acid (2013), J. Lipid Res., 54, 214-222.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.29 expressed in HEK-293 cells Mus musculus
2.7.8.29 gene PTDSS2, functional recombinant overexpression of FLAG-tagged PSS2 isozyme in HEK-293 and CHO-K1 cells Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.29 endoplasmic reticulum
-
 Mus musculus 5783
-
2.7.8.29 membrane
-
 Mus musculus 16020
-
2.7.8.29 membrane integral membrane protein Mus musculus 16020
-
2.7.8.29 microsome
-
 Mus musculus
-
-
2.7.8.29 mitochondrial membrane
-
 Mus musculus 31966
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.29 50000
-
-
 Mus musculus
2.7.8.29 50000
-
 x * 50000, calculated from amino acid sequence Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.29 L-1-phosphatidylcholine + L-serine Mus musculus isozyme PSS1 L-1-phosphatidylserine + choline
-
 r
2.7.8.29 L-1-phosphatidylethanolamine + L-serine Mus musculus isozyme PSS2 L-1-phosphatidylserine + ethanolamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.29 Mus musculus
-
-
-
2.7.8.29 Mus musculus Q99LH2
-
-
2.7.8.29 Mus musculus Q9Z1X2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.29 anti-FLAG M2 affinity gel chromatography Mus musculus
2.7.8.29 purification of functional recombinant FLAG-tagged PSS2 from HEK-293 and CHO-K1 cell microsomes by ultracentrifugation, immunoaffinity chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.29 brain
-
 Mus musculus
-
2.7.8.29 additional information isozyme PSS1 is ubiquitously expressed Mus musculus
-
2.7.8.29 additional information isozyme PSS2 is expressed in a tissue-specific manner. It is highly expressed in tissues such as brain and testis, where docosahexaenoic acid (DHA, 22:6n-3) is also highly enriched Mus musculus
-
2.7.8.29 Purkinje neuron
-
 Mus musculus
-
2.7.8.29 Sertoli cell
-
 Mus musculus
-
2.7.8.29 testis
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.29 1,2-diacylphosphatidylethanolamine + L-serine the substrate is 6times better utilized than phosphatidylethanolamine plasmalogen Mus musculus ?
-
 ?
2.7.8.29 L-1-phosphatidylcholine + L-serine isozyme PSS1 Mus musculus L-1-phosphatidylserine + choline
-
 r
2.7.8.29 L-1-phosphatidylethanolamine + L-serine
-
 Mus musculus L-1-phosphatidylserine + ethanolamine
-
 ?
2.7.8.29 L-1-phosphatidylethanolamine + L-serine isozyme PSS2 Mus musculus L-1-phosphatidylserine + ethanolamine
-
 r
2.7.8.29 additional information the enzyme synthesizes docosahexaenoic acid-phosphatidylserine much more efficiently than phosphatidylserine containing either 18:1 or 20:4 at the sn-2 position. Although no sn-1 fatty acyl preference is noted, phosphatidylserine synthase 2 exhibits significant preference toward docosahexaenoic acid compared with 18:1 or 20:4 at the sn-2 position Mus musculus ?
-
 ?
2.7.8.29 additional information isozyme PSS1 shows a 2fold higher preference for sn-1 18:0 compared with 16:0 when the sn-2 position contains 18:1 Mus musculus ?
-
 ?
2.7.8.29 additional information the recombinant FLAG-tagged enzyme is also active with phosphatidylethanolamine plasmalogen, [C18(plasm),22:6-PS], which is six times less effective as a substrate than the corresponding ester-linked phosphatidylethanolamine species, the isozyme is active with oleic acid (OA; 18:1n-9), DHA (DHA; 22:6n-3), or arachidonic acid esters. Compounds analysis by mass spectroscopy. A carbonyl group next to the glycerol backbone in the substrate-binding pocket substantially increases the catalytic activity of PSS2. Isozyme PSS2 shows a 10fold preference for sn-1 18:0, when 22:6 is esterified at the sn-2 position. Synthesis of 18:0,22:6-PS is most efficient compared with 18:0,18:1-PS and with 18:0,20:4-PS. Substrate specificity and acyl chain preference of isozyme PS2, overview Mus musculus ?
-
 ?
2.7.8.29 phosphatidylethanolamine plasmalogen + L-serine
-
 Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.29 ? x * 50000, calculated from amino acid sequence Mus musculus
2.7.8.29 ? x * 50000, about, recombinant FLAG-tagged PSS2, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.8.29 phosphatidylserine synthase 1
-
 Mus musculus
2.7.8.29 phosphatidylserine synthase 2
-
 Mus musculus
2.7.8.29 PS synthase 1
-
 Mus musculus
2.7.8.29 PS synthase 2
-
 Mus musculus
2.7.8.29 PSS1
-
 Mus musculus
2.7.8.29 PSS2
-
 Mus musculus
2.7.8.29 PTDSS2
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.29 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.29 7.4
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.7.8.29 metabolism phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2 Mus musculus
2.7.8.29 physiological function the enzyme plays a key role in phosphatidylserine accumulation in brain and testis through high activity toward docosahexaenoic acid-containing substrates Mus musculus
2.7.8.29 physiological function isozyme PSS2 may play a key role in phosphatidylserine accumulation in brain and testis through high activity toward DHA-containing substrates that are abundant in these tissues Mus musculus