BRENDA - Enzyme Database

Purification and characterization of cytoplasmic NADP+-isocitrate dehydrogenase, and amplification of the NADP+-IDH gene from the wing-dimorphic sand field cricket, Gryllus firmus

Zera, A.J.; Newman, S.; Berkheim, D.; Black, C.; Klug, L.; Crone, E.; J. Insect Sci. 11, 53 (2011)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
additional information
-
additional information
Michaelis-Menten kinetics
Gryllus firmus
1.1.1.42
0.0023
-
NADP+
pH 8.0, 28C
Gryllus firmus
1.1.1.42
0.0147
-
isocitrate
pH 8.0, 28C
Gryllus firmus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytoplasm
-
Gryllus firmus
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
required
Gryllus firmus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
46300
-
x * 46300, SDS-PAGE
Gryllus firmus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Gryllus firmus
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Gryllus firmus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
native enzyme by PEG fractionation, affinity and hydrophobic interaction chromatography
Gryllus firmus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
fat body
-
Gryllus firmus
-
1.1.1.42
gut
-
Gryllus firmus
-
1.1.1.42
head
-
Gryllus firmus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
722896
Gryllus firmus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
?
x * 46300, SDS-PAGE
Gryllus firmus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
28
-
assay at
Gryllus firmus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8
-
assay at
Gryllus firmus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Gryllus firmus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Gryllus firmus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
additional information
-
additional information
Michaelis-Menten kinetics
Gryllus firmus
1.1.1.42
0.0023
-
NADP+
pH 8.0, 28C
Gryllus firmus
1.1.1.42
0.0147
-
isocitrate
pH 8.0, 28C
Gryllus firmus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytoplasm
-
Gryllus firmus
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
required
Gryllus firmus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
46300
-
x * 46300, SDS-PAGE
Gryllus firmus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Gryllus firmus
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
native enzyme by PEG fractionation, affinity and hydrophobic interaction chromatography
Gryllus firmus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
fat body
-
Gryllus firmus
-
1.1.1.42
gut
-
Gryllus firmus
-
1.1.1.42
head
-
Gryllus firmus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
722896
Gryllus firmus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
?
x * 46300, SDS-PAGE
Gryllus firmus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
28
-
assay at
Gryllus firmus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8
-
assay at
Gryllus firmus
General Information
EC Number
General Information
Commentary
Organism
1.1.1.42
physiological function
in the cytoplasm, NADP+-IDH often contributes significantly to the NADPH pool required for reductive fatty acid biosynthesis
Gryllus firmus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.42
physiological function
in the cytoplasm, NADP+-IDH often contributes significantly to the NADPH pool required for reductive fatty acid biosynthesis
Gryllus firmus