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Literature summary extracted from

  • Zera, A.J.; Newman, S.; Berkheim, D.; Black, C.; Klug, L.; Crone, E.
    Purification and characterization of cytoplasmic NADP+-isocitrate dehydrogenase, and amplification of the NADP+-IDH gene from the wing-dimorphic sand field cricket, Gryllus firmus (2011), J. Insect Sci., 11, 53.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 additional information
-
 additional information Michaelis-Menten kinetics Gryllus firmus
1.1.1.42 0.0023
-
 NADP+ pH 8.0, 28°C Gryllus firmus
1.1.1.42 0.0147
-
 isocitrate pH 8.0, 28°C Gryllus firmus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytoplasm
-
 Gryllus firmus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ required Gryllus firmus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 46300
-
 x * 46300, SDS-PAGE Gryllus firmus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Gryllus firmus
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Gryllus firmus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 native enzyme by PEG fractionation, affinity and hydrophobic interaction chromatography Gryllus firmus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.42 fat body
-
 Gryllus firmus
-
1.1.1.42 gut
-
 Gryllus firmus
-
1.1.1.42 head
-
 Gryllus firmus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Gryllus firmus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 ? x * 46300, SDS-PAGE Gryllus firmus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 NADP+-IDH
-
 Gryllus firmus
1.1.1.42 NADP+-isocitrate dehydrogenase
-
 Gryllus firmus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 28
-
 assay at Gryllus firmus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 8
-
 assay at Gryllus firmus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Gryllus firmus

General Information

EC Number General Information Comment Organism
1.1.1.42 physiological function in the cytoplasm, NADP+-IDH often contributes significantly to the NADPH pool required for reductive fatty acid biosynthesis Gryllus firmus