Literature summary extracted from

Demir, A.S.; Talpur, F.N.; Betul Sopaci, S.; Kohring, G.W.; Celik, A.
Selective oxidation and reduction reactions with cofactor regeneration mediated by galactitol-, lactate-, and formate dehydrogenases immobilized on magnetic nanoparticles (2011), J. Biotechnol., 152, 176-183.

Application

EC Number	Application	Comment	Organism
1.1.1.406	synthesis	synthesis of (S)-1,2-propanediol and L-tagatose starting from hydroxyacetone and galactitol using an immobilized enzyme system. One-pot purification of His6-tagged GatDH and FDH followed by the production of rare sugar and chiral diol by use of affinity magnetic nanoparticles	Cereibacter sphaeroides

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.406	expression in Escherichia coli strain BL21GOLD(DE3)	Cereibacter sphaeroides

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.1.1.406	additional information	rapid immobilization with the one-pot purification of galactitol dehydrogenase and formate dehydrogenase, using iminodiacetic acid with chelated Co2+ modified magnetic nanoparticles as a carrier, for (S)-1,2-propanediol and L-tagatose production. Lactate dehydrogenase from recombinant Escherichia coli and formate dehydrogenase from Candida methylica are used for the regeneration of NADH/NAD+ with a representative synthesis of (S)-1,2-propanediol and L-tagatose starting from hydroxyacetone and galactitol, overview. The immobilized enzyme system retained up to 70% of its activity after one week of repeated use. One-pot purification of His6-tagged GatDH and FDH followed by the production of rare sugar and chiral diol by use of affinity magnetic nanoparticles	Cereibacter sphaeroides

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.406	Cereibacter sphaeroides	C0KTJ6	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.406	recombinant His(6)-tagged GatDH from Escherichia coli strain BL21GOLD(DE3) by use of Co2+-IDA functionalized Fe2O3 nanoparticles, purification and immobilization method, overview	Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.406	galactitol + NAD+	-	Cereibacter sphaeroides	L-tagatose + NADH + H+	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.406	GatDH	-	Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.406	30	-	assay at	Cereibacter sphaeroides

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.406	6.5	-	assay at	Cereibacter sphaeroides

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.406	NAD+	-	Cereibacter sphaeroides
1.1.1.406	NADH	-	Cereibacter sphaeroides

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Release 2023.1 (January 2023)