BRENDA - Enzyme Database

Identification of pantoate kinase and phosphopantothenate synthetase from Methanospirillum hungatei

Katoh, H.; Tamaki, H.; Tokutake, Y.; Hanada, S.; Chohnan, S.; J. Biosci. Bioeng. 115, 372-376 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.2.36
acetyl-CoA
activates enzyme activity 1.64fold
Methanospirillum hungatei
6.3.2.36
CoA
activates enzyme activity 3.12fold
Methanospirillum hungatei
6.3.2.36
malonyl-CoA
activates enzyme activity 1.36fold
Methanospirillum hungatei
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.7.1.169
expression in Escherichia coli
Methanospirillum hungatei
6.3.2.36
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, introduced with pSC101-based plasmid encoding argU, ileY, and leuW tRNA genes, subcloning in Escherichia coli strain JM109
Methanospirillum hungatei
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.169
malonyl-CoA
0.2 mM, 83% residual activity
Methanospirillum hungatei
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.169
Ca2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
Co2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
Fe2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
Mg2+
maximum activity in presence of Mg2+
Methanospirillum hungatei
2.7.1.169
Mn2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
additional information
not accepted: Cu2+
Methanospirillum hungatei
2.7.1.169
Ni2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
Zn2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
6.3.2.36
Mg2+
required
Methanospirillum hungatei
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.3.2.36
ATP + (R)-4-phosphopantoate + beta-alanine
Methanospirillum hungatei
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
6.3.2.36
ATP + (R)-4-phosphopantoate + beta-alanine
Methanospirillum hungatei JF-1
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.1.169
Methanospirillum hungatei
Q2FUB2
-
-
6.3.2.36
Methanospirillum hungatei
Q2FUA9
-
-
6.3.2.36
Methanospirillum hungatei JF-1
Q2FUA9
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.3.2.36
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3) by nickel affinity chromatography, dialysis and ultrafiltration
Methanospirillum hungatei
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.1.169
ATP + (R)-pantoate
-
722842
Methanospirillum hungatei
ADP + (R)-phosphopantoate
-
-
-
?
2.7.1.169
UTP + (R)-pantoate
52.7% of the activity with ATP
722842
Methanospirillum hungatei
UDP + (R)-phosphopantoate
-
-
-
?
6.3.2.36
ATP + (R)-4-phosphopantoate + beta-alanine
-
722842
Methanospirillum hungatei
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
6.3.2.36
ATP + (R)-4-phosphopantoate + beta-alanine
-
722842
Methanospirillum hungatei JF-1
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.1.169
COG1829
-
Methanospirillum hungatei
2.7.1.169
Mhun_0831
-
Methanospirillum hungatei
6.3.2.36
Mhun_0832
-
Methanospirillum hungatei
6.3.2.36
phosphopantothenate synthetase
-
Methanospirillum hungatei
6.3.2.36
Pps
-
Methanospirillum hungatei
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.169
40
-
-
Methanospirillum hungatei
6.3.2.36
30
-
assay at
Methanospirillum hungatei
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.1.169
60
-
no residual activity
Methanospirillum hungatei
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.169
8.5
-
-
Methanospirillum hungatei
6.3.2.36
7.5
-
assay at
Methanospirillum hungatei
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.169
8
9.5
more than 80% of maximum activity
Methanospirillum hungatei
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.3.2.36
ATP
-
Methanospirillum hungatei
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.2.36
acetyl-CoA
activates enzyme activity 1.64fold
Methanospirillum hungatei
6.3.2.36
CoA
activates enzyme activity 3.12fold
Methanospirillum hungatei
6.3.2.36
malonyl-CoA
activates enzyme activity 1.36fold
Methanospirillum hungatei
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.169
expression in Escherichia coli
Methanospirillum hungatei
6.3.2.36
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, introduced with pSC101-based plasmid encoding argU, ileY, and leuW tRNA genes, subcloning in Escherichia coli strain JM109
Methanospirillum hungatei
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.3.2.36
ATP
-
Methanospirillum hungatei
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.169
malonyl-CoA
0.2 mM, 83% residual activity
Methanospirillum hungatei
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.169
Ca2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
Co2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
Fe2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
Mg2+
maximum activity in presence of Mg2+
Methanospirillum hungatei
2.7.1.169
Mn2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
additional information
not accepted: Cu2+
Methanospirillum hungatei
2.7.1.169
Ni2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
2.7.1.169
Zn2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
6.3.2.36
Mg2+
required
Methanospirillum hungatei
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.3.2.36
ATP + (R)-4-phosphopantoate + beta-alanine
Methanospirillum hungatei
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
6.3.2.36
ATP + (R)-4-phosphopantoate + beta-alanine
Methanospirillum hungatei JF-1
-
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.36
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3) by nickel affinity chromatography, dialysis and ultrafiltration
Methanospirillum hungatei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.1.169
ATP + (R)-pantoate
-
722842
Methanospirillum hungatei
ADP + (R)-phosphopantoate
-
-
-
?
2.7.1.169
UTP + (R)-pantoate
52.7% of the activity with ATP
722842
Methanospirillum hungatei
UDP + (R)-phosphopantoate
-
-
-
?
6.3.2.36
ATP + (R)-4-phosphopantoate + beta-alanine
-
722842
Methanospirillum hungatei
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
6.3.2.36
ATP + (R)-4-phosphopantoate + beta-alanine
-
722842
Methanospirillum hungatei JF-1
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.169
40
-
-
Methanospirillum hungatei
6.3.2.36
30
-
assay at
Methanospirillum hungatei
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.1.169
60
-
no residual activity
Methanospirillum hungatei
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.169
8.5
-
-
Methanospirillum hungatei
6.3.2.36
7.5
-
assay at
Methanospirillum hungatei
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.169
8
9.5
more than 80% of maximum activity
Methanospirillum hungatei
General Information
EC Number
General Information
Commentary
Organism
2.7.1.169
physiological function
coexpression of genes Mhun_0831 and Mhun_0832 complements the poor growth of the temperature-sensitive Escherichia coli pantothenate kinase mutant ts9. The recombinant Mhun_0831 and Mhun_0832 genes expressed in Escherichia coli cells exhibit pantoate kinase and phosphopantothenate synthetase activities, respectively
Methanospirillum hungatei
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.169
physiological function
coexpression of genes Mhun_0831 and Mhun_0832 complements the poor growth of the temperature-sensitive Escherichia coli pantothenate kinase mutant ts9. The recombinant Mhun_0831 and Mhun_0832 genes expressed in Escherichia coli cells exhibit pantoate kinase and phosphopantothenate synthetase activities, respectively
Methanospirillum hungatei