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Literature summary extracted from

  • Nakamura, T.; Kashima, Y.; Mine, S.; Oku, T.; Uegaki, K.
    Characterization and crystal structure of the thermophilic ROK hexokinase from Thermus thermophilus (2012), J. Biosci. Bioeng., 114, 150-154.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.1 crystal structure of the enzyme is determined at a resolution of 2.02 A, with Rcryst and Rfree values of 18.1% and 22.6%, respectively Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.28
-
 D-glucose pH 7.0, 50°C Thermus thermophilus
2.7.1.1 0.37
-
 D-mannose pH 7.0, 50°C Thermus thermophilus
2.7.1.1 105
-
 D-galactose pH 7.0, 50°C Thermus thermophilus
2.7.1.1 1510
-
 D-fructose pH 7.0, 50°C Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Thermus thermophilus Q5SLJ4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 68
-
 pH 7.0, 50°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + D-fructose
-
 Thermus thermophilus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 ATP + D-galactose
-
 Thermus thermophilus ADP + D-galactose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose
-
 Thermus thermophilus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-mannose
-
 Thermus thermophilus ADP + D-mannose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 homotetramer
-
 Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 ROK hexokinase
-
 Thermus thermophilus
2.7.1.1 TthHK
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 50
-
 assay at Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.1 90
-
 TthHK is stable at 90°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 7
-
 assay at Thermus thermophilus