Literature summary extracted from

Kurata, A.; Fujita, M.; Mowafy, A.M.; Kamachi, H.; Kurihara, T.; Esaki, N.
Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase (2008), J. Biosci. Bioeng., 105, 429-431.

Application

EC Number	Application	Comment	Organism
1.3.1.103	synthesis	construction of a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9%e.e. (S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides	Burkholderia sp.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.3.1.103	expression in Escherichia coli	Burkholderia sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.3.1.103	Burkholderia sp.	Q59I44	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.3.1.103	2-chloroacrylate + NADPH + H+	no activity with NADH	Burkholderia sp.	(S)-2-chloropropanoate + NADP+	-	?

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Release 2023.1 (January 2023)