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Literature summary extracted from

  • Sha, S.; Zhou, Y.; Xin, Y.; Ma, Y.
    Development of a colorimetric assay and kinetic analysis for Mycobacterium tuberculosis D-glucose-1-phosphate thymidylyltransferase (2012), J. Biomol. Screen., 17, 252-257.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.24 analysis microtiter plate–based colorimetric assay for RmlA enzyme activity, based on hydrolysis of the diphosphate formed and detection of phosphate by malachite green reagent Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.24 expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.24 0.02
-
 dTTP pH 7.5, 37°C Mycobacterium tuberculosis
2.7.7.24 0.069
-
 alpha-D-glucose 1-phosphate pH 7.5, 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.24 Mg2+ required, optimal concentration 5 mM Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.24 33670
-
 x * 33700, SDS-PAGE, x * 33670, calculated Mycobacterium tuberculosis
2.7.7.24 33700
-
 x * 33700, SDS-PAGE, x * 33670, calculated Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.24 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.24 dTTP + alpha-D-glucose 1-phosphate
-
 Mycobacterium tuberculosis diphosphate + dTDP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.24 ? x * 33700, SDS-PAGE, x * 33670, calculated Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.24 RmlA
-
 Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.24 37
-
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.24 7.5
-
-
 Mycobacterium tuberculosis