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Literature summary extracted from

  • Kaminski, P.A.; Labesse, G.
    Phosphodeoxyribosyltransferases: designed enzymes for deoxyribonucleotide synthesis (2013), J. Biol. Chem., 288, 6534-6541.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.6
-
 Lactobacillus leichmannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.6 11
-
 2'-deoxycytidine mutant F13R/D92S/N123S, pH 6.0, 37°C Lactobacillus leichmannii
2.4.2.6 22
-
 2'-deoxycytidine mutant F13R/D92S, pH 6.0, 37°C Lactobacillus leichmannii

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.6 Lactobacillus leichmannii Q9R5V5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.6 2'-deoxyadenosine + 5-fluorouracil reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S Lactobacillus leichmannii 2'-deoxy-D-ribosyl-5-fluorouracil + adenine
-
 ?
2.4.2.6 2'-deoxycytidine + 2,6-diaminopurine reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S Lactobacillus leichmannii 2,6-diaminopurine 2-deoxy-D-riboside + cytosine
-
 ?
2.4.2.6 2'-deoxycytidine + 5-bromouracil reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S Lactobacillus leichmannii 2'-deoxy-5-bromouridine + cytosine
-
 ?
2.4.2.6 2'-deoxycytidine + 6-chloropurine reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S Lactobacillus leichmannii 6-chloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
-
 ?
2.4.2.6 2'-deoxycytidine + adenine
-
 Lactobacillus leichmannii 2'-deoxyadenosine + cytosine
-
 ?
2.4.2.6 dCMP + 2,6-dichloropurine reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S Lactobacillus leichmannii 2,6-dichloropurin-9-yl-2'-deoxy-beta-D-ribose 5'-phosphate + cytosine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.6 0.3
-
 2'-deoxycytidine mutant F13R/D92S, pH 6.0, 37°C Lactobacillus leichmannii
2.4.2.6 2.1
-
 2'-deoxycytidine mutant F13R/D92S/N123S, pH 6.0, 37°C Lactobacillus leichmannii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.6 0.0136
-
 2'-deoxycytidine mutant F13R/D92S, pH 6.0, 37°C Lactobacillus leichmannii
2.4.2.6 0.192
-
 2'-deoxycytidine mutant F13R/D92S/N123S, pH 6.0, 37°C Lactobacillus leichmannii