BRENDA - Enzyme Database

Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator

Shulga, Y.V.; Anderson, R.A.; Topham, M.K.; Epand, R.M.; J. Biol. Chem. 287, 35953-35963 (2012)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
1,2-diarachidonoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1,2-dilinoleoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1,2-dioleoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1-arachidoyl-2-arachidonoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1-stearoyl-2-arachidonoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1-stearoyl-2-oleoyl phosphatidic acid
-
Mus musculus
2.7.1.68
additional information
PIP5K activation depends on the acyl chain composition of both substrate and activator
Mus musculus
2.7.1.68
phosphatidic acid
-
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.68
expression of HA-tagged PIP5K isozymes in COS-7 cells, expression of isozyme PIP5Kalpha mutant D322A
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.68
D322A
isozyme PIP5Kalpha mutant that shows reduced activity compared to the wild-type enzyme
Mus musculus
2.7.1.68
L202I
the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme
Mus musculus
2.7.1.68
L210I
the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.68
1.6
-
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kgamma, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
2.8
-
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kalpha, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
3.7
-
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kbeta, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
4.9
-
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kbeta, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
15
-
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kgamma, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
16
-
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kalpha, pH 7.5, temperature not specified in the publication
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.68
membrane
isozyme PIP5Kalpha
Mus musculus
16020
-
2.7.1.68
nucleus
isozyme PIP5Kalpha
Mus musculus
5634
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.68
Mg2+
required
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Mus musculus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.68
Mus musculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.68
muscle cell
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1,2-dipalmitoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
722784
Mus musculus
ADP + 1,2-dipalmitoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
722784
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate
-
722784
Mus musculus
ADP + 1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate
-
722784
Mus musculus
ADP + 1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.68
7.5
-
assay at
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.68
ATP
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
1,2-diarachidonoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1,2-dilinoleoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1,2-dioleoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1-arachidoyl-2-arachidonoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1-stearoyl-2-arachidonoyl phosphatidic acid
-
Mus musculus
2.7.1.68
1-stearoyl-2-oleoyl phosphatidic acid
-
Mus musculus
2.7.1.68
additional information
PIP5K activation depends on the acyl chain composition of both substrate and activator
Mus musculus
2.7.1.68
phosphatidic acid
-
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.68
expression of HA-tagged PIP5K isozymes in COS-7 cells, expression of isozyme PIP5Kalpha mutant D322A
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.68
ATP
-
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.68
D322A
isozyme PIP5Kalpha mutant that shows reduced activity compared to the wild-type enzyme
Mus musculus
2.7.1.68
L202I
the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme
Mus musculus
2.7.1.68
L210I
the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.68
1.6
-
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kgamma, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
2.8
-
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kalpha, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
3.7
-
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kbeta, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
4.9
-
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kbeta, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
15
-
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kgamma, pH 7.5, temperature not specified in the publication
Mus musculus
2.7.1.68
16
-
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate
isozyme PIP5Kalpha, pH 7.5, temperature not specified in the publication
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.68
membrane
isozyme PIP5Kalpha
Mus musculus
16020
-
2.7.1.68
nucleus
isozyme PIP5Kalpha
Mus musculus
5634
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.68
Mg2+
required
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Mus musculus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.68
muscle cell
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1,2-dipalmitoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
722784
Mus musculus
ADP + 1,2-dipalmitoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
722784
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate
-
722784
Mus musculus
ADP + 1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate
-
722784
Mus musculus
ADP + 1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.68
7.5
-
assay at
Mus musculus