Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Endo-Streeter, S.; Tsui, M.K.; Odom, A.R.; Block, J.; York, J.D.
    Structural studies and protein engineering of inositol phosphate multikinase (2012), J. Biol. Chem., 287, 35360-35369.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.151 isoform inositol phosphate multikinase alpha, to 2.9 A resolution. The structural core of Arabidopsis thaliana IPMK is homologous to those of Saccharamyces cerevisiae IPMK and Homo sapiens IP3K Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.151 K117W mutant exhibits nearly normal 6-kinase function but harbors significantly reduced 3-kinase activity. The mutant complements conditional nutritional growth defects observed in ipmk null yeast and suppresses lethality observed in ipmk null flies Arabidopsis thaliana
2.7.1.151 K117W/K121W mutant exhibits nearly normal 6-kinase function but harbors significantly reduced 3-kinase activity. The mutant complements conditional nutritional growth defects observed in ipmk null yeast and suppresses lethality observed in ipmk null flies Arabidopsis thaliana
2.7.1.151 K121W although no inositol tetrakisphosphate is present with wild-type IPMK, levels of inositol tetrakisphosphate and inositol hexakisphosphate are equivalent in mutant K121W Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.151 0.0308
-
 1D-myo-inositol 1,3,4,5-tetrakisphosphate wild-type, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 0.05
-
 1D-myo-inositol 1,4,5-trisphosphate mutant K121W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 0.075
-
 1D-myo-inositol 1,4,5,6-tetrakisphosphate wild-type, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 0.162
-
 1D-myo-inositol 1,4,5-trisphosphate wild-type, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 0.216
-
 1D-myo-inositol 1,4,5,6-tetrakisphosphate mutant K121W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 0.254
-
 1D-myo-inositol 1,4,5,6-tetrakisphosphate mutant K117W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 0.49
-
 1D-myo-inositol 1,3,4,5-tetrakisphosphate mutant K121W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 0.568
-
 1D-myo-inositol 1,3,4,5-tetrakisphosphate mutant K117W, pH 6.6, 30°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.151 Arabidopsis thaliana Q9LY23 isoform inositol phosphate multikinase alpha
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.151 ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.151 Arg82
-
 Arabidopsis thaliana
2.7.1.151 Ipk2a
-
 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.151 0.97
-
 1D-myo-inositol 1,4,5,6-tetrakisphosphate mutant K121W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 2.04
-
 1D-myo-inositol 1,4,5,6-tetrakisphosphate mutant K117W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 5.8
-
 1D-myo-inositol 1,3,4,5-tetrakisphosphate mutant K117W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 7.3
-
 1D-myo-inositol 1,3,4,5-tetrakisphosphate mutant K121W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 29.7
-
 1D-myo-inositol 1,4,5-trisphosphate pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 37.8
-
 1D-myo-inositol 1,4,5-trisphosphate mutant K121W, pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 79.1
-
 1D-myo-inositol 1,4,5,6-tetrakisphosphate pH 6.6, 30°C Arabidopsis thaliana
2.7.1.151 106
-
 1D-myo-inositol 1,3,4,5-tetrakisphosphate pH 6.6, 30°C Arabidopsis thaliana