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Literature summary extracted from

  • Hurd, T.R.; Collins, Y.; Abakumova, I.; Chouchani, E.T.; Baranowski, B.; Fearnley, I.M.; Prime, T.A.; Murphy, M.P.; James, A.M.
    Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species (2012), J. Biol. Chem., 287, 35153-35160.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.2 expression of PDHK2 in HEK293 cells as FLAG-tagged proteins and under a tetracycline-inducible system Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.2 C195A site-directed mutagenesis Rattus norvegicus
2.7.11.2 C212A site-directed mutagenesis Rattus norvegicus
2.7.11.2 C395A site-directed mutagenesis Rattus norvegicus
2.7.11.2 C45A site-directed mutagenesis Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.2 Dichloroacetate
-
 Rattus norvegicus
2.7.11.2 H2O2 PDHK2 activity is inhibited by low levels of hydrogen peroxide generated by the respiratory chain via reversible oxidation of Cys45 and Cys392 on PDHK2 Rattus norvegicus
2.7.11.2 menadione PDHK2 activity is inhibited via reversible oxidation of Cys45 and Cys392 on PDHK2 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.2 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.2 100000
-
 dimeric enzyme, an intermolecular disulfide between two Cys-392 residues is present, in wild-type enzyme and mutant C45A Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.2 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.2 heart
-
 Rattus norvegicus
-

Subunits

EC Number Subunits Comment Organism
2.7.11.2 dimer
-
 Rattus norvegicus
2.7.11.2 monomer
-
 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.2 PDHK2
-
 Rattus norvegicus
2.7.11.2 pyruvate dehydrogenase kinase 2
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.11.2 malfunction enzyme inhibition results in increased pyruvate dehydrogenase complex activity. Oxidation of Cys45 or Cys392 inhibits PDHK2 activity. Dimer formation is not essential for PDHK2 inactivation via Cys392 oxidation, although it may potentiate it Rattus norvegicus
2.7.11.2 metabolism PDHK2 is responsible for short-term metabolic responses Rattus norvegicus