EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.32 | expressed in Escherichia coli Rosetta cells and in Saccharomyces cerevisiae KS106 cells | Toxoplasma gondii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.32 | dimethylethanolamine | - |
Toxoplasma gondii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | 0.77 | - |
choline | in 100 mM Tris-HCl (pH 8.5), at 37°C | Toxoplasma gondii | |
2.7.1.32 | 0.94 | - |
dimethylethanolamine | in 100 mM Tris-HCl (pH 8.5), at 37°C | Toxoplasma gondii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.32 | cytosol | - |
Toxoplasma gondii | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 44000 | - |
x * 44000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE | Toxoplasma gondii |
2.7.1.32 | 53000 | - |
x * 53000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE | Toxoplasma gondii |
2.7.1.32 | 70000 | - |
x * 70000, full-length enzyme, SDS-PAGE | Toxoplasma gondii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.32 | Toxoplasma gondii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.32 | Ni-NTA column chromatography | Toxoplasma gondii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.32 | tachyzoite | - |
Toxoplasma gondii | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.32 | ATP + choline | - |
Toxoplasma gondii | ADP + phosphocholine | - |
? | |
2.7.1.32 | ATP + dimethylethanolamine | - |
Toxoplasma gondii | ADP + phosphodimethylethanolamine | - |
? | |
2.7.1.32 | ATP + ethanolamine | - |
Toxoplasma gondii | ADP + phosphoethanolamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.32 | ? | x * 44000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE | Toxoplasma gondii |
2.7.1.32 | ? | x * 53000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE | Toxoplasma gondii |
2.7.1.32 | ? | x * 70000, full-length enzyme, SDS-PAGE | Toxoplasma gondii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.32 | malfunction | the parasite compensates for the loss of full-length enzyme by two potential 53- and 44 kDa isoforms | Toxoplasma gondii |