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Literature summary extracted from

  • Sampels, V.; Hartmann, A.; Dietrich, I.; Coppens, I.; Sheiner, L.; Striepen, B.; Herrmann, A.; Lucius, R.; Gupta, N.
    Conditional mutagenesis of a novel choline kinase demonstrates plasticity of phosphatidylcholine biogenesis and gene expression in Toxoplasma gondii (2012), J. Biol. Chem., 287, 16289-16299.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 expressed in Escherichia coli Rosetta cells and in Saccharomyces cerevisiae KS106 cells Toxoplasma gondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 dimethylethanolamine
-
Toxoplasma gondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 0.77
-
choline in 100 mM Tris-HCl (pH 8.5), at 37°C Toxoplasma gondii
2.7.1.32 0.94
-
dimethylethanolamine in 100 mM Tris-HCl (pH 8.5), at 37°C Toxoplasma gondii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.32 cytosol
-
Toxoplasma gondii 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.32 44000
-
x * 44000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE Toxoplasma gondii
2.7.1.32 53000
-
x * 53000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE Toxoplasma gondii
2.7.1.32 70000
-
x * 70000, full-length enzyme, SDS-PAGE Toxoplasma gondii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Toxoplasma gondii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32 Ni-NTA column chromatography Toxoplasma gondii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.32 tachyzoite
-
Toxoplasma gondii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
Toxoplasma gondii ADP + phosphocholine
-
?
2.7.1.32 ATP + dimethylethanolamine
-
Toxoplasma gondii ADP + phosphodimethylethanolamine
-
?
2.7.1.32 ATP + ethanolamine
-
Toxoplasma gondii ADP + phosphoethanolamine
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.32 ? x * 44000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE Toxoplasma gondii
2.7.1.32 ? x * 53000, the parasite has two potential 53000 and 44000 Da isoforms, SDS-PAGE Toxoplasma gondii
2.7.1.32 ? x * 70000, full-length enzyme, SDS-PAGE Toxoplasma gondii

General Information

EC Number General Information Comment Organism
2.7.1.32 malfunction the parasite compensates for the loss of full-length enzyme by two potential 53- and 44 kDa isoforms Toxoplasma gondii