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Literature summary extracted from

  • Mencarelli, C.; Bode, G.H.; Losen, M.; Kulharia, M.; Molenaar, P.C.; Veerhuis, R.; Steinbusch, H.W.; De Baets, M.H.; Nicolaes, G.A.; Martinez-Martinez, P.
    Goodpasture antigen-binding protein/ceramide transporter binds to human serum amyloid P-component and is present in brain amyloid plaques (2012), J. Biol. Chem., 287, 14897-14911.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.9 expression of FLAG-tagged Goodpasture antigen-binding protein in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.9 I439A/W588A site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P Homo sapiens
2.7.11.9 additional information immobilization of Goodpasture antigen-binding protein by human serum amyloid P component binding from solution and in plasma, overview. Serum amyloid P associates in the blood with a 37-kDa GPBP fragment containing the N-terminal region and the middle domain Homo sapiens
2.7.11.9 N460A/Y461A site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P Homo sapiens
2.7.11.9 P500G/A501Q site-directed mutagenesis, the mutant binding to serum amyloid P is abolished Homo sapiens
2.7.11.9 V498A/W499A site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P Homo sapiens
2.7.11.9 Y461A/F462A site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.9 extracellular matrix
-
 Homo sapiens 31012
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.9 Mg2+ required Homo sapiens
2.7.11.9 additional information binding of collagen IV to Goodpasture antigen-binding protein is independent of the presence of Ca2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.9 ATP + [Goodpasture antigen-binding protein] Homo sapiens the Goodpasture antigen-binding protein performs phosphorylation of collagen IV ADP + [Goodpasture antigen-binding phosphoprotein]
-
 ?
2.7.11.9 additional information Homo sapiens Goodpasture antigen-binding protein binds to human serum amyloid P component specifically in its physiological conformations, i.e. pentamers and decamers. Collagen IV binds to serum amyloid P and to Goodpasture antigen-binding protein, the latter also binds laminin, binding studies and kinetics, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.9 Homo sapiens Q9Y5P4 isoform 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.9 recombinant FLAG-tagged Goodpasture antigen-binding protein from Escherichia coli strain BL21(DE3) by affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.9 brain Goodpasture antigen-binding protein is present in brain amyloid plaques Homo sapiens
-
2.7.11.9 plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.9 ATP + [Goodpasture antigen-binding protein]
-
 Homo sapiens ADP + [Goodpasture antigen-binding phosphoprotein]
-
 ?
2.7.11.9 ATP + [Goodpasture antigen-binding protein] the Goodpasture antigen-binding protein performs phosphorylation of collagen IV Homo sapiens ADP + [Goodpasture antigen-binding phosphoprotein]
-
 ?
2.7.11.9 additional information Goodpasture antigen-binding protein binds to human serum amyloid P component specifically in its physiological conformations, i.e. pentamers and decamers. Collagen IV binds to serum amyloid P and to Goodpasture antigen-binding protein, the latter also binds laminin, binding studies and kinetics, overview Homo sapiens ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.9 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.11.9 physiological function Goodpasture antigen-binding protein, GPBP, is a nonconventional Ser/Thr kinase for basement membrane type IV collagen Homo sapiens