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Literature summary extracted from

  • Yoneda, K.; Sakuraba, H.; Araki, T.; Ohshima, T.
    Crystal structure of binary and ternary complexes of archaeal UDP-galactose 4-epimerase-like L-threonine dehydrogenase from Thermoplasma volcanium (2012), J. Biol. Chem., 287, 12966-12974.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.103 additional information L-ThrDH is unaffected by EDTA, Li2SO4, MgCl2, MnCl2, CaCl2, NiCl2, CoCl2, BaCl2, HgCl2, CdSO4, CuSO4, ZnCl2, or iodoacetic acid, each at 1 mM Thermoplasma volcanium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.103 overexpression in Escherichia coli Thermoplasma volcanium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.103 purified wild-type enzyme or enzyme mutant Y137F in complex with NAD+ and L-3-hydroxynorvaline or pyruvate or L-threonine, sitting drop vapor diffusion method, mixing of 0.001 ml of 40 mg/ml wild-type or 25 mg/ml mutant enzyme, and 1 mM NAD+ mixed with 0.001 ml of 100 mM cacodylate buffer, pH 6.4, 50% v/v 2-methyl-2,4-pentandiol, and 5% w/v PEG 8000, 20°C, crystals of ligand-bound wild-type or mutant enzymes by soaking the crystals in reservoir solution containing 0.3 M pyruvate for 2 h, or 0.1ML-threonine for 8 h or 0.1MDL-3-hydroxynorvaline for 7 h, respectively, X-ray diffraction structure determination and analysis at 1.77-2.07 A resolution, molecular replacement Thermoplasma volcanium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.103 additional information L-ThrDH is unaffected by EDTA, Li2SO4, MgCl2, MnCl2, CaCl2, NiCl2, CoCl2, BaCl2, HgCl2, CdSO4, CuSO4, ZnCl2, or iodoacetic acid, each at 1 mM Thermoplasma volcanium
1.1.1.103 pyruvate 20% inhibition at 10 mM Thermoplasma volcanium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 0.185
-
 NAD+ pH 10.0, 70°C Thermoplasma volcanium
1.1.1.103 17.5
-
 L-threonine pH 10.0, 70°C Thermoplasma volcanium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 35000
-
 2 * 35000, SDS-PAGE, 2 * 35843, sequence calculation Thermoplasma volcanium
1.1.1.103 35843
-
 2 * 35000, SDS-PAGE, 2 * 35843, sequence calculation Thermoplasma volcanium
1.1.1.103 77000
-
 gel filtration Thermoplasma volcanium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.103 L-threonine + NAD+ Thermoplasma volcanium
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Thermoplasma volcanium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.103 L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+ reaction mechanism involving catalytic residues T112, Y137, and K141, overview Thermoplasma volcanium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 L-threonine + NAD+
-
 Thermoplasma volcanium (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.103 dimer 2 * 35000, SDS-PAGE, 2 * 35843, sequence calculation Thermoplasma volcanium

Synonyms

EC Number Synonyms Comment Organism
1.1.1.103 L-ThrDH
-
 Thermoplasma volcanium
1.1.1.103 L-threonine dehydrogenase
-
 Thermoplasma volcanium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.103 70
-
-
 Thermoplasma volcanium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.103 70
-
 purified recombinant enzyme, 10 min, 90% activity remaining Thermoplasma volcanium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.103 10
-
-
 Thermoplasma volcanium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.103 4.5 9.5 purified recombinant enzyme, 20 min, 50°C, no loss in activity Thermoplasma volcanium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+
-
 Thermoplasma volcanium

General Information

EC Number General Information Comment Organism
1.1.1.103 additional information active site structure and subunit interaction, overview Thermoplasma volcanium