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Literature summary extracted from

  • Dodson, H.C.; Morris, M.T.; Morris, J.C.
    Glycerol 3-phosphate alters Trypanosoma brucei hexokinase activity in response to environmental change (2011), J. Biol. Chem., 286, 33150-33157.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.1 glycerol 3-phosphate influences enzyme activity at pH 6.5 by preventing substrate and product inhibition by ATP and ADP, respectively Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 expressed as a His-tagged fusion protein in Escherichia coli Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP
-
 Trypanosoma brucei
2.7.1.1 quercetin quercetin inhibition, but not binding, is relieved by glycerol 3-phosphate at pH 6.5 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.22
-
 ATP pH 6.5, temperature not specified in the publication Trypanosoma brucei
2.7.1.1 0.28
-
 ATP pH 7.4, temperature not specified in the publication Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 using affinity and ion-exchange chromatography Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + D-glucose
-
 Trypanosoma brucei ADP + D-glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 hexokinase
-
 Trypanosoma brucei
2.7.1.1 TbHK1
-
 Trypanosoma brucei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.1 6.5
-
 enzyme shows no activity Trypanosoma brucei
2.7.1.1 7
-
 50% activity Trypanosoma brucei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.1 additional information
-
 hexokinase activity is pH-sensitive, but glycerol 3-phosphate prevents inactivation even maintaining detectable enzyme activity at pH 4.0 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.1 4.2
-
 pH 6.5, temperature not specified in the publication Trypanosoma brucei ADP
2.7.1.1 10
-
 pH 7.4, temperature not specified in the publication Trypanosoma brucei ADP