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Literature summary extracted from

  • Yoshida, A.; Tomita, T.; Kuzuyama, T.; Nishiyama, M.
    Mechanism of concerted inhibition of alpha2beta2-type hetero-oligomeric aspartate kinase from Corynebacterium glutamicum (2010), J. Biol. Chem., 285, 27477-27486.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 expressed in Escherichia coli as a His-tagged fusion protein Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.4 D45A the inhibition of CgAK by lysine is substantially reduced in D45A mutant Corynebacterium glutamicum
2.7.2.4 S301F the S301F mutant exhibits resistance to feedback inhibition by lysine and threonine, showing activity in the presence of both lysine and threonine Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-lysine enzyme is inhibited by lysine and threonine in a concerted manner: comparison of the crystal structures between inhibitory and active forms reveal that binding inhibitors causes a conformational change to a closed inhibitory form, and the interaction between the catalytic domain in the alpha subunit and beta subunit Corynebacterium glutamicum
2.7.2.4 L-threonine enzyme is inhibited by lysine and threonine in a concerted manner: comparison of the crystal structures between inhibitory and active forms reveal that binding inhibitors causes a conformational change to a closed inhibitory form, and the interaction between the catalytic domain in the alpha subunit and beta subunit Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4 using Ni-NTA chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Corynebacterium glutamicum ADP + 4-phospho-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.4 heterotetramer crystal structure, heterotetramer composed of two alpha subunits and two beta subunits Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 CgAK
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.4 30
-
 assay at Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.4 7.5
-
 assay at Corynebacterium glutamicum