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Literature summary extracted from

  • Boitz, J.M.; Ullman, B.
    Amplification of adenine phosphoribosyltransferase suppresses the conditionally lethal growth and virulence phenotype of Leishmania donovani mutants lacking both hypoxanthine-guanine and xanthine phosphoribosyltransferases (2010), J. Biol. Chem., 285, 18555-18564.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.7 0.0012
-
 adenine pH 7.4, temperature not specified in the publication Leishmania donovani
2.4.2.7 1.5
-
 hypoxanthine pH 7.4, temperature not specified in the publication Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Leishmania donovani
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Leishmania donovani AMP + diphosphate
-
 ?
2.4.2.7 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate at about 5.7% of the conversion rate for adenine Leishmania donovani IMP + diphosphate
-
 ?

General Information

EC Number General Information Comment Organism
2.4.2.7 physiological function amplification of adenine phosphoribosyltransferase suppresses the conditionally lethal growth and virulence phenotype of Leishmania donovani mutants lacking both hypoxanthine-guanine and xanthine phosphoribosyltransferases. Transfection of mutants lacking both hypoxanthine-guanine and xanthine phosphoribosyltransferases with an adenine phosphoribosyltransferase episome recapitulates the suppressor phenotype in vitro and enables growth on 6-oxypurines. Hypoxanthine is an inefficient substrate for adenine phosphoribosyltransferase Leishmania donovani

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.7 0.00035
-
 hypoxanthine pH 7.4, temperature not specified in the publication Leishmania donovani
2.4.2.7 1090
-
 adenine pH 7.4, temperature not specified in the publication Leishmania donovani