Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bailey, S.; Rapson, T.; Johnson-Winters, K.; Astashkin, A.V.; Enemark, J.H.; Kappler, U.
    Molecular basis for enzymatic sulfite oxidation: how three conserved active site residues shape enzyme activity (2009), J. Biol. Chem., 284, 2053-2063.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.2.1 crystal structure Starkeya novella

Protein Variants

EC Number Protein Variants Comment Organism
1.8.2.1 H57A mutant with reduced activity, Tyr-236 and His-57 are necessary to stabilize Arg-55 in a position for optimal hydrogen bonding to the heme 6-propionate Starkeya novella
1.8.2.1 R55M mutant with reduced activity, R-55 is an important position close to the substrate binding site, where it makes hydrogen bonds to the equatorial oxo ligand of the molybdenum, to Gln-33, and a nearby water molecule. It also forms a salt bridge, comprising two hydrogen bonds, with propionate-6 of the heme moiety of the cytochrome subunit Starkeya novella
1.8.2.1 Y236F mutant with reduced activity, Tyr-236 and His-57 are necessary to stabilize Arg-55 in a position for optimal hydrogen bonding to the heme 6-propionate Starkeya novella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.1 0.000004
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F Starkeya novella
1.8.2.1 0.000007
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F Starkeya novella
1.8.2.1 0.0006
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme Starkeya novella
1.8.2.1 0.0011
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme Starkeya novella
1.8.2.1 0.0037
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme Starkeya novella
1.8.2.1 0.0071
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme Starkeya novella
1.8.2.1 0.022
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme Starkeya novella
1.8.2.1 0.026
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F Starkeya novella
1.8.2.1 0.042
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F Starkeya novella
1.8.2.1 0.086
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme Starkeya novella
1.8.2.1 0.114
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F Starkeya novella
1.8.2.1 0.189
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A Starkeya novella
1.8.2.1 0.22
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A Starkeya novella
1.8.2.1 0.27
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A Starkeya novella
1.8.2.1 0.29
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A Starkeya novella
1.8.2.1 0.324
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme Starkeya novella
1.8.2.1 0.332
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236Fyme Starkeya novella
1.8.2.1 0.452
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A Starkeya novella
1.8.2.1 0.667
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A Starkeya novella
1.8.2.1 0.812
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M Starkeya novella
1.8.2.1 1.087
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M Starkeya novella
1.8.2.1 1.155
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F Starkeya novella
1.8.2.1 1.46
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A Starkeya novella
1.8.2.1 1.63
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M Starkeya novella
1.8.2.1 1.66
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme Starkeya novella
1.8.2.1 1.95
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M Starkeya novella
1.8.2.1 3.39
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme Starkeya novella
1.8.2.1 3.62
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M Starkeya novella
1.8.2.1 4.456
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F Starkeya novella
1.8.2.1 8.17
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M Starkeya novella
1.8.2.1 12.2
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A Starkeya novella
1.8.2.1 15.49
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F Starkeya novella

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.1 Molybdenum
-
 Starkeya novella

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.2.1 sulfite + ferricytochrome c + H2O Starkeya novella
-
 sulfate + ferrocytochrome c + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Starkeya novella Q9LA16
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricytochrome c + H2O
-
 Starkeya novella sulfate + ferrocytochrome c + H+
-
 ?
1.8.2.1 sulfite + ferricytochrome c + H2O cytochrome c from horse heart Starkeya novella sulfate + ferrocytochrome c + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.2.1 SDH
-
 Starkeya novella

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.2.1 2 3.7 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme Starkeya novella
1.8.2.1 36.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F Starkeya novella
1.8.2.1 41.6
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F Starkeya novella
1.8.2.1 48.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F Starkeya novella
1.8.2.1 51.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F Starkeya novella
1.8.2.1 52.7
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F Starkeya novella
1.8.2.1 53.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F Starkeya novella
1.8.2.1 58.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236F Starkeya novella
1.8.2.1 63.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme Starkeya novella
1.8.2.1 64
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M Starkeya novella
1.8.2.1 64
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M Starkeya novella
1.8.2.1 64
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F Starkeya novella
1.8.2.1 66.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M Starkeya novella
1.8.2.1 68.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M Starkeya novella
1.8.2.1 70.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M Starkeya novella
1.8.2.1 73.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M Starkeya novella
1.8.2.1 86.2
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme Starkeya novella
1.8.2.1 88.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F Starkeya novella
1.8.2.1 148.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A Starkeya novella
1.8.2.1 158.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme Starkeya novella
1.8.2.1 192.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A Starkeya novella
1.8.2.1 214.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A Starkeya novella
1.8.2.1 214.6
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A Starkeya novella
1.8.2.1 220.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A Starkeya novella
1.8.2.1 222.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A Starkeya novella
1.8.2.1 226.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A Starkeya novella
1.8.2.1 238.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A Starkeya novella
1.8.2.1 293.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme Starkeya novella
1.8.2.1 345.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme Starkeya novella
1.8.2.1 410
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme Starkeya novella
1.8.2.1 431
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme Starkeya novella
1.8.2.1 519
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme Starkeya novella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.1 9
-
-
 Starkeya novella

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.2.1 8 10 less than 50% of maximal activity above and below Starkeya novella

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.1 heme c
-
 Starkeya novella
1.8.2.1 molybdenum cofactor (molybdopterin-S,S)-oxo-molybdenum Starkeya novella

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.2.1 2.84
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F Starkeya novella
1.8.2.1 8.98
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M Starkeya novella
1.8.2.1 11.6
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F Starkeya novella
1.8.2.1 18.9
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M Starkeya novella
1.8.2.1 34
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M Starkeya novella
1.8.2.1 54.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F Starkeya novella
1.8.2.1 58.9
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M Starkeya novella
1.8.2.1 67.7
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme Starkeya novella
1.8.2.1 86.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M Starkeya novella
1.8.2.1 122
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A Starkeya novella
1.8.2.1 132
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A Starkeya novella
1.8.2.1 173
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236F Starkeya novella
1.8.2.1 251
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme Starkeya novella
1.8.2.1 358
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A Starkeya novella
1.8.2.1 461
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F Starkeya novella
1.8.2.1 492
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A Starkeya novella
1.8.2.1 782
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A Starkeya novella
1.8.2.1 799
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A Starkeya novella
1.8.2.1 1010
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A Starkeya novella
1.8.2.1 1130
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A Starkeya novella
1.8.2.1 1140
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F Starkeya novella
1.8.2.1 1500
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme Starkeya novella
1.8.2.1 1970
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F Starkeya novella
1.8.2.1 3930
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M Starkeya novella
1.8.2.1 4880
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme Starkeya novella
1.8.2.1 5600
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F Starkeya novella
1.8.2.1 8420
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F Starkeya novella
1.8.2.1 15300
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme Starkeya novella
1.8.2.1 40300
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme Starkeya novella
1.8.2.1 42700
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme Starkeya novella
1.8.2.1 75500
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme Starkeya novella
1.8.2.1 106000
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme Starkeya novella