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Literature summary extracted from

  • Kiyonari, S.; Takayama, K.; Nishida, H.; Ishino, Y.
    Identification of a novel binding motif in Pyrococcus furiosus DNA ligase for the functional interaction with proliferating cell nuclear antigen (2006), J. Biol. Chem., 281, 28023-28032.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.5.1.1 proliferating cell nuclear antigen stimulatory effect of Pyrococcus furiosus proliferating cell nuclear antigen (PCNA) on the enzyme activity of Pyrococcus furiosus DNA ligase is observed not at low ionic strength, but at a high salt concentration, at which a DNA ligase alone cannot bind to a nicked DNA substrate. Identification of the amino acid residues that are critical for PCNA binding in a loop structure located in the N-terminal DNA-binding domain of Ppyrococcus furiosus DNA ligase. The pentapeptide motif QKSFF is involved in the PCNA-interacting motifs, in which Gln and the first Phe are especially important for stable binding with PCNA Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expression in Escherichia coli Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.1 K249A mutant enzyme shows no no adenylyltransferase activity Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Pyrococcus furiosus P56709
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m no DNA ligase activity in presence of ADP, AMP, and NAD+ Pyrococcus furiosus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 PfuLig
-
 Pyrococcus furiosus