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Literature summary extracted from

  • Yokoyama, H.; Matsui, I.
    A novel thermostable membrane protease forming an operon with a stomatin homolog from the hyperthermophilic archaebacterium Pyrococcus horikoshii (2005), J. Biol. Chem., 280, 6588-6594.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B56 the N-terminal region of PH1510 (1510-N, residues 16–236) is expressed in Escherichia coli Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.B56 D168A mutant enzyme shows 3.2% activity with casein compared with the activity of the wild-type enzyme. The mutant enzyme shows a 25000 Da band instead of 45000 Da band Pyrococcus horikoshii
3.4.21.B56 K138A mutant enzyme shows 1.2% activity with casein compared with the activity of the wild-type enzyme Pyrococcus horikoshii
3.4.21.B56 S97A mutant enzyme shows 0.08% activity with casein compared with the activity of the wild-type enzyme Pyrococcus horikoshii
3.4.21.B56 T62A mutant enzyme shows 5.5% activity with casein compared with the activity of the wild-type enzyme Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B56 3,4-dichloroisocoumarin 1 mM, 49% inhibition Pyrococcus horikoshii
3.4.21.B56 CaCl2 0.1 M, 11% activity Pyrococcus horikoshii
3.4.21.B56 dodecyl-beta-D-maltoside 0.1%, 38% activity Pyrococcus horikoshii
3.4.21.B56 EDTA 5 mM, 67% inhibition Pyrococcus horikoshii
3.4.21.B56 iodoacetate 5 mM, 17% inhibition Pyrococcus horikoshii
3.4.21.B56 MgCl2 0.1 M, 14% residual activity Pyrococcus horikoshii
3.4.21.B56 MnCl2 0.1 M, 4% activity Pyrococcus horikoshii
3.4.21.B56 NaCl 0.1 M, 90% loss of activity Pyrococcus horikoshii
3.4.21.B56 pepstatin 0.005 mM, 21% inhibition Pyrococcus horikoshii
3.4.21.B56 phenylmethylsulfonyl fluoride 5 mM, 21% inhibition Pyrococcus horikoshii
3.4.21.B56 SDS 0.01%, 53% activity. 1% SDS, 1.2% activity Pyrococcus horikoshii
3.4.21.B56 ZnCl2 0.1 M, 9% activity Pyrococcus horikoshii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B56 25000
-
 2 * 25000, dimer of the N-terminal fragment of PH1510 (1510-N, residues 16–236), SDS-PAGE Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B56 Pyrococcus horikoshii O59179 N-terminal region of open reading frame, PH1510 (residues 16–236, designated as 1510-N) is a serine protease
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B56 purification of the N-terminal fragment of PH1510 (1510-N, residues 16–236) expressed in Escherichia coli Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B56 alpha-casein + H2O alpha-casein is not cleaved as effectively as beta-casein. 1510-N probably cleaves the substrate specifically. The cleavage sites contain many hydrophobic and aromatic residues. The 1510-N protease possibly recognizes leucine at the cleavage site Pyrococcus horikoshii ?
-
 ?
3.4.21.B56 beta-casein + H2O alpha-casein is not cleaved as effectively as beta-casein. 1510-N probably cleaves the substrate specifically. The cleavage sites contain many hydrophobic and aromatic residues. The 1510-N protease possibly recognizes leucine at the cleavage site Pyrococcus horikoshii ?
-
 ?
3.4.21.B56 additional information 1510-N is a serine protease with a catalytic residue, Ser-97 Pyrococcus horikoshii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B56 dimer 2 * 25000, dimer of the N-terminal fragment of PH1510 (1510-N, residues 16–236), SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B56 PH1510
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B56 80
-
 assay at Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B56 5 6
-
 Pyrococcus horikoshii