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Literature summary extracted from

  • Sleeman, M.C.; Sorensen, J.L.; Batchelar, E.T.; McDonough, M.A.; Schofield, C.J.
    Structural and mechanistic studies on carboxymethylproline synthase (CarB), a unique member of the crotonase superfamily catalyzing the first step in carbapenem biosynthesis (2005), J. Biol. Chem., 280, 34956-34965.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.226 expression in Escherichia coli Pectobacterium carotovorum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.226 hanging drop vapor diffusion method, crystal structure of CarB in the presence and absence of acetyl-CoA at 2.24 A and 3.15 A resolution. The structures reveal that CarB contains a conserved oxy-anion hole probably required for decarboxylation of malonyl-CoA and stabilization of the resultant enolate Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O Pectobacterium carotovorum
-
 CoA + (2S,5S)-5-carboxymethylproline + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.226 Pectobacterium carotovorum Q9XB60
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O
-
 Pectobacterium carotovorum CoA + (2S,5S)-5-carboxymethylproline + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.226 CarB
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.226 37
-
 assay at Pectobacterium carotovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.226 7.5
-
 assay at Pectobacterium carotovorum