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Literature summary extracted from

  • Sleeman, M.C.; Schofield, C.J.
    Carboxymethylproline synthase (CarB), an unusual carbon-carbon bond-forming enzyme of the crotonase superfamily involved in carbapenem biosynthesis (2004), J. Biol. Chem., 279, 6730-6736.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.226 expression in Escherichia coli Pectobacterium carotovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.226 27575
-
 3 * 27575, calculated from sequence, negative ion electrospray MS, enzyme exists predominantly in a trimeric form Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O Pectobacterium carotovorum the enzyme is involved in the biosynthesis of 1-carbapen-2-em-3-carboxylic acid, a simple beta-lactam antibiotic CoA + (2S,5S)-carboxymethylproline + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.226 Pectobacterium carotovorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.226
-
 Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.226 4
-
 pH 7.5, 37°C Pectobacterium carotovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O acetyl-CoA is not a substrate Pectobacterium carotovorum CoA + (2S,5S)-5-carboxymethylproline + CO2
-
 ?
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O the enzyme is involved in the biosynthesis of 1-carbapen-2-em-3-carboxylic acid, a simple beta-lactam antibiotic Pectobacterium carotovorum CoA + (2S,5S)-carboxymethylproline + CO2
-
 ?
2.3.1.226 methylmalonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O
-
 Pectobacterium carotovorum CoA + (2S,5S)-6-methyl-5-carboxymethylproline + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.226 trimer 3 * 27575, calculated from sequence, negative ion electrospray MS, enzyme exists predominantly in a trimeric form Pectobacterium carotovorum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.226 CarB
-
 Pectobacterium carotovorum