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Literature summary extracted from

  • Mandal, A.K.; Yang, Y.; Kertesz, T.M.; Argueello, J.M.
    Identification of the transmembrane metal binding site in Cu+-transporting PIB-type ATPases (2004), J. Biol. Chem., 279, 54802-54807.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.8 I685E Cu+/ATPase activity is about 50% of the activity of the wild-type enzyme Archaeoglobus fulgidus
7.2.2.8 I685T Cu+/ATPase activity is about 25% of the activity of the wild-type enzyme Archaeoglobus fulgidus
7.2.2.8 L686A Cu+/ATPase activity is about 80% of the activity of the wild-type enzyme Archaeoglobus fulgidus
7.2.2.8 M711A no Cu+/ATPase activity Archaeoglobus fulgidus
7.2.2.8 M711C no Cu+/ATPase activity Archaeoglobus fulgidus
7.2.2.8 additional information Tyr682, Asn683, Met711 and Ser715 are identified as required for Cu+ binding. Replacement of these residues abolishes enzyme activity. These mutant proteins do not undergo Cu+-dependent phosphorylation by ATP but are phosphorylated by inorganic phosphate in the absence of Cu+ Archaeoglobus fulgidus
7.2.2.8 N683A no Cu+/ATPase activity Archaeoglobus fulgidus
7.2.2.8 N683Q no Cu+/ATPase activity Archaeoglobus fulgidus
7.2.2.8 P688A Cu+/ATPase activity is about 70% of the activity of the wild-type enzyme Archaeoglobus fulgidus
7.2.2.8 P704A Cu+/ATPase activity is about 75% of the activity of the wild-type enzyme Archaeoglobus fulgidus
7.2.2.8 S714A Cu+/ATPase activity is about 30% of the activity of the wild-type enzyme Archaeoglobus fulgidus
7.2.2.8 S714R Cu+/ATPase activity is about 65% of the activity of the wild-type enzyme Archaeoglobus fulgidus
7.2.2.8 S715A no Cu+/ATPase activity Archaeoglobus fulgidus
7.2.2.8 S715R no Cu+/ATPase activity Archaeoglobus fulgidus
7.2.2.8 Y682A no Cu+/ATPase activity Archaeoglobus fulgidus
7.2.2.8 Y682S Cu+/ATPase activity is about 10% of the activity of the wild-type enzyme Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.8 Archaeoglobus fulgidus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.8 ATP + H2O + Cu+[side 1] Tyr682, Asn683, Met711 and Ser715 are identified as required for Cu+ binding Archaeoglobus fulgidus ADP + phosphate + Cu+[side 2]
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 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.8 CopA
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 Archaeoglobus fulgidus
7.2.2.8 Cu+-transporting ATPase
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 Archaeoglobus fulgidus