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Literature summary extracted from

  • OHandley, S.; Dunn, C.; Bessman, M.
    Orf135 from Escherichia coli is a nudix hydrolase specific for CTP, dCTP, and 5-methyl-dCTP (2001), J. Biol. Chem., 276, 5421-5426.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.65
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.65 0.172
-
 5-Methyl-dCTP pH 9.0, 37°C Escherichia coli
3.6.1.65 0.551
-
 CTP pH 9.0, 37°C Escherichia coli
3.6.1.65 0.767
-
 dCTP pH 9.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.65 Mg2+ most effective cation, with optimal activity at 5 mM Mg2+ Escherichia coli
3.6.1.65 Mn2+ about 10% of the activity with Mg2+ Escherichia coli
3.6.1.65 additional information absolute requirement for a divalnt cation. No activity in presence of Zn2+, Co2+, Ca2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.65 Escherichia coli P77788
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.65 193
-
 substrate 5-methyl-dCTP, pH 9.0, 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.65 5-methyl-dCTP + H2O
-
 Escherichia coli 5-methyl-dCMP + diphosphate
-
 ?
3.6.1.65 arabinosyl-CTP + H2O 3% of the activity with 5-methyl-dCTP Escherichia coli arabinosyl-CMP + diphosphate
-
 ?
3.6.1.65 CDP + H2O 2% of the activity with 5-methyl-dCTP Escherichia coli cytidine + diphosphate
-
 ?
3.6.1.65 CTP + H2O 48% of the activity with 5-methyl-dCTP Escherichia coli CMP + diphosphate
-
 ?
3.6.1.65 dCTP + H2O 23% of the activity with 5-methyl-dCTP Escherichia coli dCMP + diphosphate
-
 ?
3.6.1.65 dTTP + H2O 2% of the activity with 5-methyl-dCTP Escherichia coli dTMP + diphosphate
-
 ?
3.6.1.65 dUTP + H2O 1% of the activity with 5-methyl-dCTP Escherichia coli dUMP + diphosphate
-
 ?
3.6.1.65 additional information enzyme is highly specific for pyrimidine (deoxy)nucleoside triphosphates. Like other Nudix hydrolases, the enzyme cleaves its substrates to produce a nucleoside monophosphate and diphosphate Escherichia coli ?
-
 ?
3.6.1.65 additional information no substrates: CMP, dCMP, dATP, ATP, dGTP, GTP, dUTP, CDP-ethanolamine, CDP-choline, CDP-glucose, CDP-glycerol Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.65 14.3
-
 dCTP pH 9.0, 37°C Escherichia coli
3.6.1.65 25.8
-
 CTP pH 9.0, 37°C Escherichia coli
3.6.1.65 51.7
-
 5-Methyl-dCTP pH 9.0, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.65 8.5 9
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.65 18
-
 dCTP pH 9.0, 37°C Escherichia coli
3.6.1.65 47
-
 CTP pH 9.0, 37°C Escherichia coli
3.6.1.65 301
-
 5-Methyl-dCTP pH 9.0, 37°C Escherichia coli