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Literature summary extracted from

  • Shen, Y.; Musti, K.; Hiramoto, M.; Kikuchi, H.; Kawarabayashi, Y.; Matsui, I.
    Invariant Asp-1122 and Asp-1124 are essential residues for polymerization catalysis of family D DNA polymerase from Pyrococcus horikoshii (2001), J. Biol. Chem., 276, 27376-27383.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 expression in Escherichia coli Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.7 D1122A loss of polymerization activity Pyrococcus horikoshii
2.7.7.7 D1122E mutant enzyme with reduced polymerization activity, polymerization activity is 15% compared to wild-type activity, 3'-5' exonuclease activity remains, the mutant has lower Mg2+ affinity than does the wild-type Pyrococcus horikoshii
2.7.7.7 D1124A loss of polymerization activity Pyrococcus horikoshii
2.7.7.7 D1124E mutant enzyme with reduced polymerization activity, polymerization activity is 43% compared to wild-type activity, Pyrococcus horikoshii
2.7.7.7 D1124N mutant enzyme with reduced polymerization activity, 3'-5' exonuclease activity remains, the mutant has lower Mg2+ affinity than does the wild-type Pyrococcus horikoshii
2.7.7.7 G1123A mutant enzyme has 13% of the activity of the wild type enzyme Pyrococcus horikoshii
2.7.7.7 G1123R mutant enzyme has 0.8% of the activity of the wild type enzyme Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ optimal magnesium concentration is 17.5 mM Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 90000
-
 2 * 90000 + 2 * 140000, SDS-PAGE Pyrococcus horikoshii
2.7.7.7 140000
-
 2 * 90000 + 2 * 140000, SDS-PAGE Pyrococcus horikoshii
2.7.7.7 421000
-
 gel filtration Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Pyrococcus horikoshii O57863 and O57861 O57863: small subunit, O57861: large subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Pyrococcus horikoshii diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 heterotetramer 2 * 90000 + 2 * 140000, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DP1Pho small subunit Pyrococcus horikoshii
2.7.7.7 DP2Pho large subunit Pyrococcus horikoshii
2.7.7.7 PH0121 gene name, large subunit Pyrococcus horikoshii
2.7.7.7 PH0123 gene name, small subunit Pyrococcus horikoshii
2.7.7.7 PolDPho
-
 Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 80
-
 60 min, 50% loss of activity Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 8.5 9
-
 Pyrococcus horikoshii