EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.144 | 3-deoxy-D-arabinoheptulosonic acid 7-phosphate | 1 mM, 40-50% activation | Amycolatopsis mediterranei |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.144 | expression in Escherichia coli | Amycolatopsis mediterranei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.144 | 0.164 | - |
5-amino-5-deoxy-3-dehydroshikimate | pH 7.5, 28°C | Amycolatopsis mediterranei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.144 | 39000 | - |
2 * 39000, SDS-PAGE, 2 * 46101, calculated | Amycolatopsis mediterranei |
4.2.1.144 | 46101 | - |
2 * 39000, SDS-PAGE, 2 * 46101, calculated | Amycolatopsis mediterranei |
4.2.1.144 | 74000 | - |
gel filtration | Amycolatopsis mediterranei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.144 | Amycolatopsis mediterranei | O52552 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.144 | both native and recombinant protein | Amycolatopsis mediterranei |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.144 | 72 | - |
pH 7.5, 28°C, native enzyme | Amycolatopsis mediterranei |
4.2.1.144 | 195 | - |
pH 7.5, 28°C, recombinant enzyme | Amycolatopsis mediterranei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.144 | 5-amino-5-deoxy-3-dehydroshikimate | - |
Amycolatopsis mediterranei | 3-amino-5-hydroxybenzoate + H2O | - |
? | |
4.2.1.144 | additional information | no substrates: 5-deoxy-5-amino-3-dehydroquinic acid, 5-deoxy-5-aminoshikimic acid, quinic acid, 3-dehydroquinic acid, or 3-dehydroshikimic acid | Amycolatopsis mediterranei | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.144 | dimer | 2 * 39000, SDS-PAGE, 2 * 46101, calculated | Amycolatopsis mediterranei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.144 | 33 | - |
- |
Amycolatopsis mediterranei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.144 | 28 | 50 | more than 84% of maximum activity within | Amycolatopsis mediterranei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.144 | 7.5 | - |
- |
Amycolatopsis mediterranei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.144 | pyridoxal 5'-phosphate | enzyme is a dimer containing one molecule of pyridoxal phosphate per subunit. The enzyme-bound pyridoxal phosphate forms a Schiffs base with the amino group of 5-deoxy-5-amino-3-dehydroshikimic acid and catalyzes both an alpha,beta-dehydration and a stereospecific 1,4-enolization of the substrate | Amycolatopsis mediterranei |