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Literature summary extracted from

  • Taylor, S.V.; Kelleher, N.L.; Kinsland, C.; Chiu, H.J.; Costello, C.A.; Backstrom, A.D.; McLafferty, F.W.; Begley, T.P.
    Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation (1998), J. Biol. Chem., 273, 16555-16560.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.73 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.73 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.73 ATP + [sulfur-carrier protein ThiS] Escherichia coli this reaction is likely to be involved in the activation of ThiS for sulfur transfer from cysteine or from a cysteine-derived sulfur donor diphosphate + adenylyl-[sulfur-carrier protein ThiS]
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.73 Escherichia coli P30138
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.73 ammonium sulfate precipitation, DEAE column chromatography, and Superdex 75 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.73 ATP + [sulfur-carrier protein ThiS] this reaction is likely to be involved in the activation of ThiS for sulfur transfer from cysteine or from a cysteine-derived sulfur donor Escherichia coli diphosphate + adenylyl-[sulfur-carrier protein ThiS]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.73 ThiF
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.73 ATP
-
 Escherichia coli