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Literature summary extracted from

  • Silva, J.C.; Townsend, C.A.
    Heterologous expression, isolation, and characterization of versicolorin B synthase from Aspergillus parasiticus. A key enzyme in the aflatoxin B1 biosynthetic pathway (1997), J. Biol. Chem., 272, 804-813.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.143 expression in Saccharomyces cerevisiae Aspergillus parasiticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.143 1-ethyl-3(3-dimethylaminopropyl)carbodiimide 10 mM, 12% residual activity Aspergillus parasiticus
4.2.1.143 3-(2-hydroxyethyl)-2,3-dihydro-1-benzofuran-2,4-diol 0.05 mM, 30% inhibition. Compound mimics the C-ring and side chain of the substrate Aspergillus parasiticus
4.2.1.143 diethyl dicarbonate 10 mM, 41% residual activity Aspergillus parasiticus
4.2.1.143 Hg2+ 1 mM, complete loss of activity Aspergillus parasiticus
4.2.1.143 additional information not inhibitory: FAD, FMN, riboflavin, and glucose Aspergillus parasiticus
4.2.1.143 versicolorin B 0.015 mM, 50% inhibition Aspergillus parasiticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.143 0.0065
-
 versiconal pH 6.5, 37°C Aspergillus parasiticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.143 70000
-
 x * 78000, SDS-PAGE, recombinant protein, x * 70000, SDS-PAGE of PNGase F treated protein Aspergillus parasiticus
4.2.1.143 78000
-
 x * 78000, SDS-PAGE, recombinant protein, x * 70000, SDS-PAGE of PNGase F treated protein Aspergillus parasiticus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.143 Aspergillus parasiticus Q12062
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.1.143 glycoprotein sequence contains 3 potential N-glycosylation sites. Treatment with PNGase F leads to a decrease in molecular mass from 78000 kDa for wild-type to 70000 kDa Aspergillus parasiticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.143 2750
-
 pH 6.5, 37°C Aspergillus parasiticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.143 versiconal
-
 Aspergillus parasiticus versicolorin B + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.143 ? x * 78000, SDS-PAGE, recombinant protein, x * 70000, SDS-PAGE of PNGase F treated protein Aspergillus parasiticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.143 2.1
-
 versiconal pH 6.5, 37°C Aspergillus parasiticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.143 0.096
-
 3-(2-hydroxyethyl)-2,3-dihydro-1-benzofuran-2,4-diol pH 6.5, 37°C Aspergillus parasiticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.143 Aspergillus parasiticus isoelectric focusing
-
 4.6