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Literature summary extracted from

  • Watanabe, M.; Matsuo, M.; Tanaka, S.; Akimoto, H.; Asahi, S.; Nishimura, S.; Katze, J.R.; Hashizume, T.; Crain, P.F.; McCloskey, J.A.; Okada, N.
    Biosynthesis of archaeosine, a novel derivative of 7-deazaguanosine specific to archaeal tRNA, proceeds via a pathway involving base replacement on the tRNA polynucleotide chain (1997), J. Biol. Chem., 272, 20146-20151.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.48 Mg2+ Mg2+ may be responsible for conformational rigidity of the tRNA, but not for the enzymatic activity itself. Mg2+ ion is required for activity with the T7 transcript as a substrate. Activity with unfractionated Escherichia coli tRNA does not require Mg2+ Haloferax volcanii
2.4.2.48 additional information high salt concentration approximately 2.4 M is necessary for maximum activity Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.48 78000
-
 x * 78000, SDS-PAGE Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine Haloferax volcanii key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine Haloferax volcanii ATCC 29605 key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.48 Haloferax volcanii Q9C4M3
-
-
2.4.2.48 Haloferax volcanii ATCC 29605 Q9C4M3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.48
-
 Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs Haloferax volcanii 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction Haloferax volcanii 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs Haloferax volcanii ATCC 29605 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?
2.4.2.48 guanine15 in tRNA + 7-cyano-7-carbaguanine archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction Haloferax volcanii ATCC 29605 7-cyano-7-carbaguanine15 in tRNA + guanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.48 ? x * 78000, SDS-PAGE Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
2.4.2.48 tRNA-guanine transglycosylase
-
 Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.48 7.5
-
-
 Haloferax volcanii