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Literature summary extracted from

  • Dinkova-Kostova, A.T.; Gang, D.R.; Davin, L.B.; Bedgar, D.L.; Chu, A.; Lewis, N.G.
    (+)-Pinoresinol/(+)-lariciresinol reductase from Forsythia intermedia. Protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase (1996), J. Biol. Chem., 271, 29473-29482.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.23.1.1 expressed in Escherichia coli Nova-Blue cells Forsythia x intermedia
1.23.1.2 expressed in Escherichia coli Nova-Blue cells Forsythia x intermedia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.23.1.1 39400
-
 x * 39400, calculated from amino acid sequence Forsythia x intermedia
1.23.1.1 59000
-
 gel filtration Forsythia x intermedia
1.23.1.2 39400
-
 x * 39400, calculated from amino acid sequence Forsythia x intermedia
1.23.1.2 59000
-
 gel filtration Forsythia x intermedia

Organism

EC Number Organism UniProt Comment Textmining
1.23.1.1 Forsythia x intermedia
-
-
-
1.23.1.2 Forsythia x intermedia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.23.1.1 ammonium sulfate fractionation, Affinity Blue Gel filtration, phenyl Sepharose column chromatography, hydroxyapatite column chromatography, 2’,5’ ADP-Sepharose column chromatography, Affinity-Yellow gel filtration, and Superose 12 gel filtration Forsythia x intermedia
1.23.1.2 ammonium sulfate fractionation, Affinity Blue Gel filtration, phenyl Sepharose column chromatography, hydroxyapatite column chromatography, 2’,5’ ADP-Sepharose column chromatography, Affinity-Yellow gel filtration, and Superose 12 gel filtration Forsythia x intermedia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.23.1.1 stem
-
 Forsythia x intermedia
-
1.23.1.2 stem
-
 Forsythia x intermedia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.23.1.1 0.000083
-
 crude extract, at pH 7.4 and 30°C Forsythia x intermedia
1.23.1.1 0.255
-
 after 3060fold purification, at pH 7.4 and 30°C Forsythia x intermedia
1.23.1.2 0.000083
-
 crude extract, at pH 7.4 and 30°C Forsythia x intermedia
1.23.1.2 0.255
-
 after 3060fold purification, at pH 7.4 and 30°C Forsythia x intermedia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.23.1.1 (+)-pinoresinol + NADPH + H+
-
 Forsythia x intermedia (+)-lariciresinol + NADP+
-
 ?
1.23.1.2 (+)-lariciresinol + NADPH + H+
-
 Forsythia x intermedia (-)-secoisolariciresinol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.23.1.1 monomer or dimer x * 35000-36000, SDS-PAGE Forsythia x intermedia
1.23.1.1 monomer or dimer x * 39400, calculated from amino acid sequence Forsythia x intermedia
1.23.1.2 monomer or dimer x * 35000-36000, SDS-PAGE Forsythia x intermedia
1.23.1.2 monomer or dimer x * 39400, calculated from amino acid sequence Forsythia x intermedia

Synonyms

EC Number Synonyms Comment Organism
1.23.1.1 (+)-pinoresinol/(+)-lariciresinol reductase
-
 Forsythia x intermedia
1.23.1.1 PLR
-
 Forsythia x intermedia
1.23.1.2 (+)-pinoresinol/(+)-lariciresinol reductase
-
 Forsythia x intermedia
1.23.1.2 PLR
-
 Forsythia x intermedia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.23.1.1 30
-
-
 Forsythia x intermedia
1.23.1.2 30
-
-
 Forsythia x intermedia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.23.1.1 7.4
-
-
 Forsythia x intermedia
1.23.1.2 7.4
-
-
 Forsythia x intermedia

Cofactor

EC Number Cofactor Comment Organism Structure
1.23.1.1 NADPH dependent on Forsythia x intermedia
1.23.1.2 NADPH dependent on Forsythia x intermedia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.23.1.1 Forsythia x intermedia isoelectric focusing
-
 5.7
1.23.1.1 Forsythia x intermedia calculated from amino acid sequence
-
 7.1
1.23.1.2 Forsythia x intermedia isoelectric focusing
-
 5.7
1.23.1.2 Forsythia x intermedia calculated from amino acid sequence
-
 7.1

General Information

EC Number General Information Comment Organism
1.23.1.1 metabolism (+)-pinoresinol/(+)-lariciresinol reductase is a pivotal branchpoint enzyme in lignan biosynthesis Forsythia x intermedia
1.23.1.2 metabolism (+)-pinoresinol/(+)-lariciresinol reductase is a pivotal branchpoint enzyme in lignan biosynthesis Forsythia x intermedia