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Literature summary extracted from

  • Keister, D.L.; San Pietro, A.; Stolzenbach, F.E.
    Pyridine nucleotide transhydrogenase from spinach. I. Purification and properties (1960), J. Biol. Chem., 235, 2989-2996.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.1.3 2'-adenylic acid 43% inhibition, activity with NADPH and NAD+ Spinacia oleracea
1.6.1.3 3'-adenylic acid 37% inhibition, activity with NADPH and NAD+ Spinacia oleracea
1.6.1.3 5'-adenylic acid 27% inhibition, activity with NADPH and NAD+ Spinacia oleracea
1.6.1.3 ADP 48% inhibition, activity with NADPH and NAD+ Spinacia oleracea
1.6.1.3 Alpha-NAD+ 33% inhibition, activity with NADPH and NAD+ Spinacia oleracea
1.6.1.3 ATP 22% inhibition, activity with NADPH and NAD+ Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.1.3 Mn2+ 1 mM, slight stimulation Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.1.3 NADPH + NAD+ Spinacia oleracea the enzyme catalyses the NADPH-driven reduction of NAD+ NADP+ + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.3 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.1.3
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.1.3 leaf
-
 Spinacia oleracea
-

Storage Stability

EC Number Storage Stability Organism
1.6.1.3 -15°C, partially purified, stable for at least one month Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.3 acetylpyridine-NADPH + NAD+ stereospecificity with respect to NADPH is not known Spinacia oleracea acetylpyridine-NADP+ + NADH
-
 ?
1.6.1.3 deamino-NADPH + NAD+ stereospecificity with respect to NADPH is not known Spinacia oleracea deamino-NADP+ + NADH
-
 ?
1.6.1.3 NADPH + acetylpyridine-NAD+ stereospecificity with respect to NADPH is not known Spinacia oleracea NADP+ + acetylpyridine-NADH
-
 ?
1.6.1.3 NADPH + deamino-NAD+ stereospecificity with respect to NADPH is not known Spinacia oleracea NADP+ + deamino-NADH
-
 ?
1.6.1.3 NADPH + NAD+ the enzyme catalyses the NADPH-driven reduction of NAD+ Spinacia oleracea NADP+ + NADH
-
 ?
1.6.1.3 NADPH + NAD+ stereospecificity with respect to NADPH is not known Spinacia oleracea NADP+ + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.1.3 22
-
 assay at room temperature Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.1.3 22
-
 there is no loss in activity when the enzyme is left overnight at room temperature Spinacia oleracea
1.6.1.3 55
-
 2.5 min, at least 90% of the activity is retained Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.1.3 8.8
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.1.3 7.6 9.4 pH 7.6: about 40% of maximal activity, pH 9.4: about 70% of maximal activity Spinacia oleracea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.6.1.3 4
-
 no loss in activity after 15 min, 45% loss of activity after 4 hours Spinacia oleracea