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Literature summary extracted from

  • Machtey, M.; Kuhn, M.L.; Flasch, D.A.; Aleanzi, M.; Ballicora, M.A.; Iglesias, A.A.
    Insights into glycogen metabolism in chemolithoautotrophic bacteria from distinctive kinetic and regulatory properties of ADP-glucose pyrophosphorylase from Nitrosomonas europaea (2012), J. Bacteriol., 194, 6056-6065.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 oxaloacetate
-
 Nitrosomonas europaea
2.7.7.27 phosphoenolpyruvate
-
 Nitrosomonas europaea
2.7.7.27 pyruvate
-
 Nitrosomonas europaea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 expression in Escherichia coli Nitrosomonas europaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 AMP
-
 Nitrosomonas europaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.11
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, presence of Mg2+ Nitrosomonas europaea
2.7.7.27 0.13
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, presence of Co2+ Nitrosomonas europaea
2.7.7.27 0.13
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, presence of Mn2+ Nitrosomonas europaea
2.7.7.27 0.72
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, presence of Cd2+ Nitrosomonas europaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Cd2+ S0.5 value 0.74 mM in direction of synthesis of ADP-glucose Nitrosomonas europaea
2.7.7.27 Co2+ S0.5 value 0.46 mM in direction of synthesis of ADP-glucose Nitrosomonas europaea
2.7.7.27 Mg2+ S0.5 value above 40 mM in direction of synthesis of ADP-glucose Nitrosomonas europaea
2.7.7.27 Mn2+ S0.5 value 1.37 mM in direction of synthesis of ADP-glucose Nitrosomonas europaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 49000
-
 4 * 49200, calculated, 4 * 49000, SDS-PAGE Nitrosomonas europaea
2.7.7.27 49200
-
 4 * 49200, calculated, 4 * 49000, SDS-PAGE Nitrosomonas europaea
2.7.7.27 200000
-
 gel filtration Nitrosomonas europaea

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Nitrosomonas europaea Q82T88
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 recombinant protein Nitrosomonas europaea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 20
-
 pH 8.0, 37°C Nitrosomonas europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate the catalytic efficiency for ATP is not dependent upon the divalent metal ion Nitrosomonas europaea diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-mannose 1-phosphate the catalytic efficiency with glucose 1-phosphate is at least one order of magnitude higher than that with mannose 1-phosphate Nitrosomonas europaea diphosphate + ADP-mannose
-
 r
2.7.7.27 additional information enzyme is able to accept UTP, CTP, dTTP and GTP with catalytic efficiencies below 1% of that with ATP Nitrosomonas europaea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 tetramer 4 * 49200, calculated, 4 * 49000, SDS-PAGE Nitrosomonas europaea

Synonyms

EC Number Synonyms Comment Organism
2.7.7.27 GlgC
-
 Nitrosomonas europaea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27
-
 50 fully active between 0°C and 50°C for 15 min Nitrosomonas europaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 9
-
-
 Nitrosomonas europaea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.27 4.8 9.1 stable for 30 min at 37°C Nitrosomonas europaea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.27 Nitrosomonas europaea calculated
-
 5.8