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Literature summary extracted from

  • Tomita, H.; Yokooji, Y.; Ishibashi, T.; Imanaka, T.; Atomi, H.
    Biochemical characterization of pantoate kinase, a novel enzyme necessary for coenzyme A biosynthesis in the Archaea (2012), J. Bacteriol., 194, 5434-5443.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.169 expression in Thermococcus kodakarensis Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.169 D143 residue is the base responsible for proton abstraction from the pantoate hydroxy group Thermococcus kodakarensis
2.7.1.169 E134A residue is involved in binding with Mg2+ Thermococcus kodakarensis
2.7.1.169 H131A residue is involved in pantoate recognition. 77% of wild-type activity in presence of 6 mM pantoate and 4 mM ATP Thermococcus kodakarensis
2.7.1.169 R155A residue is involved in pantoate recognition and plays an important role in catalysis Thermococcus kodakarensis
2.7.1.169 S104A residue is involved in binding with phosphate Thermococcus kodakarensis
2.7.1.169 S28A residue is involved in pantoate recognition. 3.7% of wild-type activity in presence of 6 mM pantoate and 4 mM ATP Thermococcus kodakarensis
2.7.1.169 T186A residue is involved in pantoate recognition and plays an important role in catalysis. Thr186 is involved in dimer assambly Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.169 additional information not inhibitory: CoA, acetyl-CoA Thermococcus kodakarensis
2.7.1.169 Pantoate substrate inhibition Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.169 0.17
-
 UTP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 0.32
-
 ATP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 0.34
-
 CTP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 0.43
-
 GTP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 2.92
-
 Pantoate pH 7.5, 42°C Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.169 K+ moderate stimulation, optimum at 10 mM Thermococcus kodakarensis
2.7.1.169 Mg2+ required Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.169 Thermococcus kodakarensis Q5JHF1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.169 ATP + pantoate reaction displays Michaelis-Menten kinetics toward ATP, whereas substrate inhibition is observed with pantoate Thermococcus kodakarensis ADP + 4-phosphopantoate
-
 ?
2.7.1.169 CTP + pantoate
-
 Thermococcus kodakarensis CDP + 4-phosphopantoate
-
 ?
2.7.1.169 GTP + pantoate
-
 Thermococcus kodakarensis GDP + 4-phosphopantoate
-
 ?
2.7.1.169 additional information enzyme displays broad nucleotide specificity and utilizes ATP, GTP, UTP, and CTP with comparable kcat/Km values Thermococcus kodakarensis ?
-
 ?
2.7.1.169 UTP + pantoate
-
 Thermococcus kodakarensis UDP + 4-phosphopantoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.169 PoK
-
 Thermococcus kodakarensis
2.7.1.169 TK2141
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.169 80
-
-
 Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.169 0.32
-
 GTP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 0.47
-
 CTP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 1.11
-
 UTP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 1.48
-
 ATP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 2.82
-
 Pantoate pH 7.5, 42°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.169 7.5
-
-
 Thermococcus kodakarensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.169 9.75
-
 Pantoate pH 7.5, 42°C Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.169 0.74
-
 GTP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 1.4
-
 CTP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 2.2
-
 ATP pH 7.5, 42°C Thermococcus kodakarensis
2.7.1.169 6.5
-
 UTP pH 7.5, 42°C Thermococcus kodakarensis