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Literature summary extracted from

  • Noinaj, N.; Wattanasak, R.; Lee, D.Y.; Wally, J.L.; Piszczek, G.; Chock, P.B.; Stadtman, T.C.; Buchanan, S.K.
    Structural insights into the catalytic mechanism of Escherichia coli selenophosphate synthetase (2012), J. Bacteriol., 194, 499-508.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.3 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.9.3 mutant enzyme C17S in apo form, hanging drop vapor diffusion method, using 50 mM Tris-HCl (pH 8.25), 50 mM MgCl2, and 32% (w/v) PEG MME 550 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.3 D227A inactive Escherichia coli
2.7.9.3 D51A inactive Escherichia coli
2.7.9.3 D68A inactive Escherichia coli
2.7.9.3 D91A inactive Escherichia coli
2.7.9.3 N87A inactive Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.3 K+ dependent on Escherichia coli
2.7.9.3 Mg2+ dependent on Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Escherichia coli P16456
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.3 Ni-agarose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide + H2O
-
 Escherichia coli AMP + selenophosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.3 dimer sedimentation velocity analysis Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.9.3 selenophosphate synthetase
-
 Escherichia coli
2.7.9.3 SPS
-
 Escherichia coli