BRENDA - Enzyme Database

Identification and characterization of a putative arginine kinase homolog from Myxococcus xanthus required for fruiting body formation and cell differentiation

Bragg, J.; Rajkovic, A.; Anderson, C.; Curtis, R.; Van Houten, J.; Begres, B.; Naples, C.; Snider, M.; Fraga, D.; Singer, M.; J. Bacteriol. 194, 2668-2676 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.3.3
expressed in Escherichia coli BL21(DE3) cells
Myxococcus xanthus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
2.1
-
Nomega-phospho-L-Arg
at pH 8.0 and 25°C
Myxococcus xanthus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Myxococcus xanthus
Q1DA50
-
-
2.7.3.3
Myxococcus xanthus DK 1622
Q1DA50
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.3
MBPTrap HP column chromatography
Myxococcus xanthus
Storage Stability
EC Number
Storage Stability
Organism
2.7.3.3
0°C, purified enzyme on ice, 24 h, 75% loss of activity
Myxococcus xanthus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
722542
Myxococcus xanthus
ADP + Nomega-phospho-L-Arg
-
-
-
r
2.7.3.3
ATP + L-Arg
-
722542
Myxococcus xanthus DK 1622
ADP + Nomega-phospho-L-Arg
-
-
-
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.093
-
Nomega-phospho-L-Arg
at pH 8.0 and 25°C
Myxococcus xanthus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
expressed in Escherichia coli BL21(DE3) cells
Myxococcus xanthus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
2.1
-
Nomega-phospho-L-Arg
at pH 8.0 and 25°C
Myxococcus xanthus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
MBPTrap HP column chromatography
Myxococcus xanthus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.3.3
0°C, purified enzyme on ice, 24 h, 75% loss of activity
Myxococcus xanthus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
722542
Myxococcus xanthus
ADP + Nomega-phospho-L-Arg
-
-
-
r
2.7.3.3
ATP + L-Arg
-
722542
Myxococcus xanthus DK 1622
ADP + Nomega-phospho-L-Arg
-
-
-
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.093
-
Nomega-phospho-L-Arg
at pH 8.0 and 25°C
Myxococcus xanthus
General Information
EC Number
General Information
Commentary
Organism
2.7.3.3
physiological function
the putative arginine kinase from Myxococcus xanthus is required for fruiting body formation and cell differentiation
Myxococcus xanthus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.3.3
physiological function
the putative arginine kinase from Myxococcus xanthus is required for fruiting body formation and cell differentiation
Myxococcus xanthus