EC Number | Cloned (Comment) | Organism |
---|---|---|
1.16.1.9 | expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.16.1.9 | 0.033 | - |
Fe(III)-EDTA | at pH 7.5 and 37°C | Escherichia coli | |
1.16.1.9 | 0.043 | - |
NADPH | at pH 7.5 and 37°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.16.1.9 | Escherichia coli | - |
- |
- |
1.16.1.9 | Escherichia coli MG1655 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.16.1.9 | HiTrap DEAE column chromatography, Superdex 75 gel filtration | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.16.1.9 | 0.001 | - |
using ferric enterobactin as substrate, at pH 7.5 and 37°C | Escherichia coli |
1.16.1.9 | 0.022 | - |
using Fe(III)-EDTA as substrate, at pH 7.5 and 37°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.16.1.9 | Fe(III)-EDTA + NADPH + H+ | - |
Escherichia coli | Fe(II) + EDTA + NADP+ | - |
? | |
1.16.1.9 | Fe(III)-EDTA + NADPH + H+ | - |
Escherichia coli MG1655 | Fe(II) + EDTA + NADP+ | - |
? | |
1.16.1.9 | ferric enterobactin + NADPH + H+ | weak activity | Escherichia coli | ? | - |
? | |
1.16.1.9 | ferric enterobactin + NADPH + H+ | weak activity | Escherichia coli MG1655 | ? | - |
? | |
1.16.1.9 | additional information | the enzyme shows no ferric reductase activity toward ferric chloride | Escherichia coli | ? | - |
? | |
1.16.1.9 | additional information | the enzyme shows no ferric reductase activity toward ferric chloride | Escherichia coli MG1655 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.16.1.9 | ferric reductase | - |
Escherichia coli |
1.16.1.9 | YqjH | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.16.1.9 | FAD | the enzyme uses FADH2/FAD as a covalently bound cofactor rather than a substrate | Escherichia coli | |
1.16.1.9 | NADPH | dependent on | Escherichia coli |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.16.1.9 | Escherichia coli | addition of Fe2+ decreases yqjH expression starting at 100 nM, with a maximum repression of 4fold occurring at 0.01 mM Fe2+. Addition of CoCl2 concentrations of more than 500 nM also repress yqjH expression by about 50% | down |
1.16.1.9 | Escherichia coli | YqjI-dependent transcriptional repression is reduced when cells are exposed to elevated nickel levels, resulting in increased expression of yqjH. Upon addition of NiCl2, yqjH expression increases starting at 100 nM NiCl2, with maximum induction by 0.001 mM NiCl2 (42%). Deletion of yqjI leads to constitutive expression levels of yqjH that are 30fold higher than wild type basal expression levels | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.16.1.9 | malfunction | deletion of yqjH increases nickel toxicity | Escherichia coli |
1.16.1.9 | physiological function | the enzyme is required for iron homeostasis in Escherichia coli | Escherichia coli |