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Literature summary extracted from

  • Stoeveken, N.; Pittelkow, M.; Sinner, T.; Jensen, R.A.; Heider, J.; Bremer, E.
    A specialized aspartokinase enhances the biosynthesis of the osmoprotectants ectoine and hydroxyectoine in Pseudomonas stutzeri A1501 (2011), J. Bacteriol., 193, 4456-4468.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 recombinantly expressed in Escherichia coli Stutzerimonas stutzeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-lysine L-lysine inhibits Ask_LysC only in a mixture with L-threonine Stutzerimonas stutzeri
2.7.2.4 L-threonine
-
 Stutzerimonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 1.3
-
 ATP pH 8.0, 30°C Stutzerimonas stutzeri
2.7.2.4 3.8
-
 ATP pH 8.0, 30°C Stutzerimonas stutzeri
2.7.2.4 21.6
-
 L-aspartate pH 8.0, 30°C Stutzerimonas stutzeri
2.7.2.4 29.7
-
 L-aspartate pH 8.0, 30°C Stutzerimonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 18000
-
 SDS-PAGE, recombinant protein, beta-subunit (amino acids 248-255) Stutzerimonas stutzeri
2.7.2.4 44000
-
 SDS-PAGE, recombinant protein Stutzerimonas stutzeri
2.7.2.4 44300
-
 calculated from cDNA Stutzerimonas stutzeri
2.7.2.4 50000
-
 SDS-PAGE, recombinant protein Stutzerimonas stutzeri
2.7.2.4 52400
-
 calculated from cDNA Stutzerimonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Stutzerimonas stutzeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4
-
 Stutzerimonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Stutzerimonas stutzeri ADP + 4-phospho-L-aspartate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 Ask_Ect
-
 Stutzerimonas stutzeri
2.7.2.4 Ask_LysC
-
 Stutzerimonas stutzeri
2.7.2.4 aspartokinase
-
 Stutzerimonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.4 30
-
 assay at Stutzerimonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.4 8
-
 assay at Stutzerimonas stutzeri

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.2.4 0.4
-
 pH 8.0, 30°C, mixture with L-lysine Stutzerimonas stutzeri L-threonine
2.7.2.4 0.7
-
 pH 8.0, 30°C, + 650 mM NaCl Stutzerimonas stutzeri L-threonine
2.7.2.4 2.9
-
 pH 8.0, 30°C Stutzerimonas stutzeri L-threonine
2.7.2.4 3.6
-
 pH 8.0, 30°C Stutzerimonas stutzeri L-threonine
2.7.2.4 3.9
-
 pH 8.0, 30°C, + 650 mM KCl Stutzerimonas stutzeri L-threonine
2.7.2.4 13.5
-
 pH 8.0, 30°C, + 650 mM KCl Stutzerimonas stutzeri L-threonine
2.7.2.4 18.7
-
 pH 8.0, 30°C, + 650 mM NaCl Stutzerimonas stutzeri L-threonine