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Literature summary extracted from

  • Story, S.V.; Shah, C.; Jenney, F.E. Jr.; Adams, M.W.
    Characterization of a novel zinc-containing, lysine-specific aminopeptidase from the hyperthermophilic archaeon Pyrococcus furiosus (2005), J. Bacteriol., 187, 2077-2083.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.15 the recombinant protein containing a polyhistidine tag at the N terminus is produced in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.15 1.4
-
 L-arginine 4-nitroanilide pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus
3.4.11.15 1.8
-
 Lys-Gly-Gly pH 8.0, 100°C, enzyme from Pyrococcus furiosus Pyrococcus furiosus
3.4.11.15 2.7
-
 Lys-Gly-Gly pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus
3.4.11.15 4
-
 L-lysine 4-nitroanilide pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus
3.4.11.15 4.6
-
 L-lysine 4-nitroanilide pH 8.0, 100°C, enzyme from Pyrococcus furiosus Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.15 cytoplasm
-
 Pyrococcus furiosus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.15 Co2+ 0.2 mM, 4fold stimulation. When the sample is preincubated with 0.2 mM Co2+ ions and subjected to gel filtration, only 10% of the activity remains, while addition of Co2+ (0.2 mM) to the reaction mixture completely restores enzyme activity. Co2+ions could not be replaced with other divalent (Ca2+, Cd2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, or Zn2+) or monovalent cations (Na+or K+) when used at concentrations of up to 0.2 mM Pyrococcus furiosus
3.4.11.15 Zinc the enzyme contains 2.0 zinc atoms per subunit Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.15 38214
-
 4 * 38214, calculated from sequence Pyrococcus furiosus
3.4.11.15 40000
-
 4 * 40000, SDS-PAGE Pyrococcus furiosus
3.4.11.15 160000
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.15 Pyrococcus furiosus Q8TZW4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.15 native and recombinant enzyme Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.15 220
-
 pH 8.0, 100°C, 0.2 mM Co2+, purified enzyme from Pyrococcus furiosus Pyrococcus furiosus
3.4.11.15 1900
-
 pH 8.0, 100°C, 0.2 mM Co2+, purified enzyme from Pyrococcus furiosus Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.15 L-arginine 4-nitroanilide + H2O 47% of the activity compared to Lys-4-nitroanilide, recombinant enzyme Pyrococcus furiosus L-arginine + 4-nitroaniline
-
 ?
3.4.11.15 L-leucine 4-nitroanilide + H2O 7% of the activity compared to Lys-4-nitroanilide, recombinant enzyme Pyrococcus furiosus L-leucine + 4-nitroaniline
-
 ?
3.4.11.15 L-lysine 4-nitroanilide + H2O
-
 Pyrococcus furiosus L-lysine + 4-nitroaniline
-
 ?
3.4.11.15 L-proline 4-nitroanilide + H2O 6% of the activity compared to Lys-4-nitroanilide, recombinant enzyme Pyrococcus furiosus L-proline + 4-nitroaniline
-
 ?
3.4.11.15 Lys-Ala + H2O
-
 Pyrococcus furiosus Lys + Ala
-
 ?
3.4.11.15 Lys-Gly-Gly + H2O
-
 Pyrococcus furiosus Lys + Gly-Gly
-
 ?
3.4.11.15 Lys-Gly-Gly-Lys + H2O
-
 Pyrococcus furiosus Lys + Gly-Gly-Lys
-
 ?
3.4.11.15 Lys-Leu + H2O
-
 Pyrococcus furiosus Lys + Leu
-
 ?
3.4.11.15 Lys-Lys-Lys + H2O
-
 Pyrococcus furiosus Lys + Lys-Lys
-
 ?
3.4.11.15 Lys-Lys-Lys-Lys + H2O
-
 Pyrococcus furiosus Lys + Lys-Lys-Lys
-
 ?
3.4.11.15 Lys-Phe + H2O
-
 Pyrococcus furiosus Lys + Phe
-
 ?
3.4.11.15 additional information no activity (or less than 5% compared to L-lysine 4-nitroanilide): L-alanine 4-nitroanilide, L-glycine 4-nitroanilide, L-histidine 4-nitroanilide, L-phenylalanine 4-nitroanilide, phenylacetyl 4-nitroanilide Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.15 tetramer 4 * 40000, SDS-PAGE Pyrococcus furiosus
3.4.11.15 tetramer 4 * 38214, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.15 lysine aminopeptidase
-
 Pyrococcus furiosus
3.4.11.15 PF1861
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.15 100
-
 activity of enzyme from Pyrococcus furiosus and recombinant enzyme increase from 40°C to 100°C Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.15 60 100 at 60°C the recombinant enzyme shows about 35% of the activity at 100°C. At 60°C the enzyme from Pyrococcus furiosus shows about 60% of the activity at 100°C Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.15 100
-
 6 h, enzyme from Pyrococcus furiosus loses 50% of its activity, recombinant enzyme loses 50% of its activity within 10 min, presence of 0.2 mM CoCl2 increases half-life of the recombinant enzyme to 6 h Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.15 305
-
 Lys-Gly-Gly pH 8.0, 100°C, enzyme from Pyrococcus furiosus Pyrococcus furiosus
3.4.11.15 769
-
 L-arginine 4-nitroanilide pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus
3.4.11.15 1260
-
 L-lysine 4-nitroanilide pH 8.0, 100°C, enzyme from Pyrococcus furiosus Pyrococcus furiosus
3.4.11.15 1460
-
 Lys-Gly-Gly pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus
3.4.11.15 1840
-
 L-lysine 4-nitroanilide pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.15 8
-
 enzyme from Pyrococcus furiosus and recombinant enzyme Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.15 169
-
 Lys-Gly-Gly pH 8.0, 100°C, enzyme from Pyrococcus furiosus Pyrococcus furiosus
3.4.11.15 274
-
 L-lysine 4-nitroanilide pH 8.0, 100°C, enzyme from Pyrococcus furiosus Pyrococcus furiosus
3.4.11.15 460
-
 L-lysine 4-nitroanilide pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus
3.4.11.15 540
-
 Lys-Gly-Gly pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus
3.4.11.15 550
-
 L-arginine 4-nitroanilide pH 8.0, 100°C, recombinant enzyme Pyrococcus furiosus