Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Itoh, H.; Shiba, T.
    Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A (2004), J. Bacteriol., 186, 5178-5181.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.33 0.27
-
AMP forward reaction of ADP synthesis, pH 8.0, 37°C Acinetobacter johnsonii
2.7.4.33 8.3
-
ADP reverse reaction of polyphosphate synthesis, pH 8.0, 37°C Acinetobacter johnsonii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.33 Mg2+ required, best at 100 mM for polyphosphate utilization/ADP synthesis and 20 mM for polyphosphate synthesis. The equilibrium ratio for the AMP and ADP formation by PAP is 1:19 in the presence of 100 mM MgCl2 and is changed to 1:9.6, i.e. 9.4% of 5 mM ADP is converted to AMP, in the presence of 20 mM MgCl2 Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.33 ADP + [phosphate]n Acinetobacter johnsonii
-
AMP + [phosphate]n+1
-
r
2.7.4.33 ADP + [phosphate]n Acinetobacter johnsonii 210A
-
AMP + [phosphate]n+1
-
r
2.7.4.33 AMP + [phosphate]n Acinetobacter johnsonii
-
ADP + [phosphate]n-1
-
r
2.7.4.33 AMP + [phosphate]n Acinetobacter johnsonii 210A
-
ADP + [phosphate]n-1
-
r
2.7.4.33 GDP + [phosphate]n Acinetobacter johnsonii
-
GMP + [phosphate]n+1
-
r
2.7.4.33 GDP + [phosphate]n Acinetobacter johnsonii 210A
-
GMP + [phosphate]n+1
-
r
2.7.4.33 GMP + [phosphate]n Acinetobacter johnsonii
-
GDP + [phosphate]n-1
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.33 Acinetobacter johnsonii Q83XD3 gene pap
-
2.7.4.33 Acinetobacter johnsonii 210A Q83XD3 gene pap
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.33 ADP + [phosphate]n
-
Acinetobacter johnsonii AMP + [phosphate]n+1
-
r
2.7.4.33 ADP + [phosphate]n
-
Acinetobacter johnsonii 210A AMP + [phosphate]n+1
-
r
2.7.4.33 AMP + [phosphate]n
-
Acinetobacter johnsonii ADP + [phosphate]n-1
-
r
2.7.4.33 AMP + [phosphate]n best substrate Acinetobacter johnsonii ADP + [phosphate]n-1
-
?
2.7.4.33 AMP + [phosphate]n
-
Acinetobacter johnsonii 210A ADP + [phosphate]n-1
-
r
2.7.4.33 AMP + [phosphate]n best substrate Acinetobacter johnsonii 210A ADP + [phosphate]n-1
-
?
2.7.4.33 CMP + [phosphate]n low activity Acinetobacter johnsonii CDP + [phosphate]n-1
-
?
2.7.4.33 GDP + [phosphate]n
-
Acinetobacter johnsonii GMP + [phosphate]n+1
-
r
2.7.4.33 GDP + [phosphate]n
-
Acinetobacter johnsonii 210A GMP + [phosphate]n+1
-
r
2.7.4.33 GMP + [phosphate]n
-
Acinetobacter johnsonii GDP + [phosphate]n-1
-
?
2.7.4.33 GMP + [phosphate]n
-
Acinetobacter johnsonii GDP + [phosphate]n-1
-
r
2.7.4.33 additional information the forward reaction of polyphosphate utilization is preferred compared to the reverse reaction of polyphosphate synthesis. ADP is preferred over GDP as a phosphate donor for poly(P) synthesis. PAP of Acinetobacter johnsonii strain 210A is a type of poly(P) kinase that uses ADP and GDP as substrates, cf. EC 2.7.4.1. Analysis of the reversibility of the PAP reaction. The equilibrium ratio for the AMP and ADP formation by PAP is 1:19 in the presence of 100 mM MgCl2 and is changed to 1:9.6, i.e. 9.4% of 5 mM ADP is converted to AMP, in the presence of 20 mM MgCl2 Acinetobacter johnsonii ?
-
?
2.7.4.33 additional information the forward reaction of polyphosphate utilization is preferred compared to the reverse reaction of polyphosphate synthesis. ADP is preferred over GDP as a phosphate donor for poly(P) synthesis. PAP of Acinetobacter johnsonii strain 210A is a type of poly(P) kinase that uses ADP and GDP as substrates, cf. EC 2.7.4.1. Analysis of the reversibility of the PAP reaction. The equilibrium ratio for the AMP and ADP formation by PAP is 1:19 in the presence of 100 mM MgCl2 and is changed to 1:9.6, i.e. 9.4% of 5 mM ADP is converted to AMP, in the presence of 20 mM MgCl2 Acinetobacter johnsonii 210A ?
-
?
2.7.4.33 UMP + [phosphate]n low activity Acinetobacter johnsonii UDP + [phosphate]n-1
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.33 polyphosphate:AMP phosphotransferase
-
Acinetobacter johnsonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.33 37
-
assay at Acinetobacter johnsonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.33 8
-
assay at Acinetobacter johnsonii