EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.172 | expressed in Escherichia coli BL21(DE3)/pLysS cells | Ralstonia sp. |
1.18.1.7 | expressed in Escherichia coli B L21(DE3)/pLysS cells | Ralstonia sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.7 | reduced [2Fe-2S] ferredoxin + NAD+ + H+ | Ralstonia sp. | - |
oxidized [2Fe-2S] ferredoxin + NADH | - |
? | |
1.18.1.7 | reduced [2Fe-2S] ferredoxin + NAD+ + H+ | Ralstonia sp. U2 | - |
oxidized [2Fe-2S] ferredoxin + NADH | - |
? | |
1.18.1.7 | reduced [2Fe-2S] ferredoxin + NADP+ + H+ | Ralstonia sp. | - |
oxidized [2Fe-2S] ferredoxin + NADPH | - |
? | |
1.18.1.7 | reduced [2Fe-2S] ferredoxin + NADP+ + H+ | Ralstonia sp. U2 | - |
oxidized [2Fe-2S] ferredoxin + NADPH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.172 | Ralstonia sp. | O52379 and O52380 | large oxygenase component and small oxagenase component | - |
1.14.13.172 | Ralstonia sp. U2 | O52379 and O52380 | large oxygenase component and small oxagenase component | - |
1.18.1.7 | Ralstonia sp. | O52378 | - |
- |
1.18.1.7 | Ralstonia sp. U2 | O52378 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.172 | 0.051 | - |
in 50 mM MES buffer, pH 6.4, at 30°C | Ralstonia sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.172 | additional information | no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone | Ralstonia sp. | ? | - |
? | |
1.14.13.172 | additional information | no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone | Ralstonia sp. U2 | ? | - |
? | |
1.14.13.172 | salicylate + NADH + H+ + O2 | - |
Ralstonia sp. | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.172 | salicylate + NADH + H+ + O2 | - |
Ralstonia sp. U2 | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.18.1.7 | reduced [2Fe-2S] ferredoxin + NAD+ + H+ | - |
Ralstonia sp. | oxidized [2Fe-2S] ferredoxin + NADH | - |
? | |
1.18.1.7 | reduced [2Fe-2S] ferredoxin + NAD+ + H+ | - |
Ralstonia sp. U2 | oxidized [2Fe-2S] ferredoxin + NADH | - |
? | |
1.18.1.7 | reduced [2Fe-2S] ferredoxin + NADP+ + H+ | - |
Ralstonia sp. | oxidized [2Fe-2S] ferredoxin + NADPH | - |
? | |
1.18.1.7 | reduced [2Fe-2S] ferredoxin + NADP+ + H+ | - |
Ralstonia sp. U2 | oxidized [2Fe-2S] ferredoxin + NADPH | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.172 | NagGH | - |
Ralstonia sp. |
1.14.13.172 | S5H | a three-component system consisting of NagGH (the oxygenase components), NagAa (ferredoxin reductase), and NagAb (ferredoxin) | Ralstonia sp. |
1.18.1.7 | ferredoxin reductase | - |
Ralstonia sp. |
1.18.1.7 | NagAa | - |
Ralstonia sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.172 | NADH | - |
Ralstonia sp. | |
1.18.1.7 | NAD+ | - |
Ralstonia sp. | |
1.18.1.7 | NADP+ | - |
Ralstonia sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.18.1.7 | metabolism | ferredoxin reductase NagAa is required for salicylate transformation | Ralstonia sp. |