BRENDA - Enzyme Database

Salicylate 5-hydroxylase from Ralstonia sp. strain U2: a monooxygenase with close relationships to and shared electron transport proteins with naphthalene dioxygenase

Zhou, N.Y.; Al-Dulayymi, J.; Baird, M.S.; Williams, P.A.; J. Bacteriol. 184, 1547-1555 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.172
expressed in Escherichia coli BL21(DE3)/pLysS cells
Ralstonia sp.
1.18.1.7
expressed in Escherichia coli B L21(DE3)/pLysS cells
Ralstonia sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.7
reduced [2Fe-2S] ferredoxin + NAD+ + H+
Ralstonia sp.
-
oxidized [2Fe-2S] ferredoxin + NADH
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NAD+ + H+
Ralstonia sp. U2
-
oxidized [2Fe-2S] ferredoxin + NADH
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NADP+ + H+
Ralstonia sp.
-
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NADP+ + H+
Ralstonia sp. U2
-
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.172
Ralstonia sp.
O52379 and O52380
large oxygenase component and small oxagenase component
-
1.14.13.172
Ralstonia sp. U2
O52379 and O52380
large oxygenase component and small oxagenase component
-
1.18.1.7
Ralstonia sp.
O52378
-
-
1.18.1.7
Ralstonia sp. U2
O52378
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.172
0.051
-
in 50 mM MES buffer, pH 6.4, at 30°C
Ralstonia sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.172
additional information
no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone
722507
Ralstonia sp.
?
-
-
-
-
1.14.13.172
additional information
no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone
722507
Ralstonia sp. U2
?
-
-
-
-
1.14.13.172
salicylate + NADH + H+ + O2
-
722507
Ralstonia sp.
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
1.14.13.172
salicylate + NADH + H+ + O2
-
722507
Ralstonia sp. U2
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
722507
Ralstonia sp.
oxidized [2Fe-2S] ferredoxin + NADH
-
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
722507
Ralstonia sp. U2
oxidized [2Fe-2S] ferredoxin + NADH
-
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NADP+ + H+
-
722507
Ralstonia sp.
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NADP+ + H+
-
722507
Ralstonia sp. U2
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.172
NADH
-
Ralstonia sp.
1.18.1.7
NAD+
-
Ralstonia sp.
1.18.1.7
NADP+
-
Ralstonia sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.172
expressed in Escherichia coli BL21(DE3)/pLysS cells
Ralstonia sp.
1.18.1.7
expressed in Escherichia coli B L21(DE3)/pLysS cells
Ralstonia sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.172
NADH
-
Ralstonia sp.
1.18.1.7
NAD+
-
Ralstonia sp.
1.18.1.7
NADP+
-
Ralstonia sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.7
reduced [2Fe-2S] ferredoxin + NAD+ + H+
Ralstonia sp.
-
oxidized [2Fe-2S] ferredoxin + NADH
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NAD+ + H+
Ralstonia sp. U2
-
oxidized [2Fe-2S] ferredoxin + NADH
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NADP+ + H+
Ralstonia sp.
-
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NADP+ + H+
Ralstonia sp. U2
-
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.172
0.051
-
in 50 mM MES buffer, pH 6.4, at 30°C
Ralstonia sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.172
additional information
no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone
722507
Ralstonia sp.
?
-
-
-
-
1.14.13.172
additional information
no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone
722507
Ralstonia sp. U2
?
-
-
-
-
1.14.13.172
salicylate + NADH + H+ + O2
-
722507
Ralstonia sp.
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
1.14.13.172
salicylate + NADH + H+ + O2
-
722507
Ralstonia sp. U2
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
722507
Ralstonia sp.
oxidized [2Fe-2S] ferredoxin + NADH
-
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
722507
Ralstonia sp. U2
oxidized [2Fe-2S] ferredoxin + NADH
-
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NADP+ + H+
-
722507
Ralstonia sp.
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
-
?
1.18.1.7
reduced [2Fe-2S] ferredoxin + NADP+ + H+
-
722507
Ralstonia sp. U2
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.18.1.7
metabolism
ferredoxin reductase NagAa is required for salicylate transformation
Ralstonia sp.
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.18.1.7
metabolism
ferredoxin reductase NagAa is required for salicylate transformation
Ralstonia sp.