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Literature summary extracted from

  • Reed, D.W.; Millstein, J.; Hartzell, P.L.
    H(2)O(2)-forming NADH oxidase with diaphorase (cytochrome) activity from Archaeoglobus fulgidus (2001), J. Bacteriol., 183, 7007-7016.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.3.3 CHAPS 0.2-0.5%, increases activity about twofold Archaeoglobus fulgidus
1.6.3.3 n-dodecyl beta-D-maltoside 0.5%, increases activity about twofold Archaeoglobus fulgidus
1.6.3.3 Triton X-100 0.1-0.5%, increases activity about twofold Archaeoglobus fulgidus
1.6.3.3 Tween 20 1.25%, increases activity about twofold Archaeoglobus fulgidus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.3.3 expression in Escherichia coli Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.3.3 CaCl2 10 mM, slightly increases activity Archaeoglobus fulgidus
1.6.3.3 Cd(CH3CH2COO-)2 10 mM, slightly increases activity Archaeoglobus fulgidus
1.6.3.3 CoCl2 10 mM, slightly increases activity Archaeoglobus fulgidus
1.6.3.3 EGTA 10 mM, slightly increases activity Archaeoglobus fulgidus
1.6.3.3 MnCl2 10 mM, slightly increases activity Archaeoglobus fulgidus
1.6.3.3 SDS 0.5%, completely inhibits the reaction Archaeoglobus fulgidus
1.6.3.3 sodium deoxycholate 0.5%, slightly decreases activity Archaeoglobus fulgidus
1.6.3.3 ZnCl2 10 mM, slightly increases activity Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.3 0.0031
-
NADH pH 6.6, 55°C Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.3.3 CuCl2 10 mM, 3-4fold stimulation Archaeoglobus fulgidus
1.6.3.3 K3Fe(CN)6 10 mM, 3-4fold stimulation Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.3 47000
-
x * 47000, SDS-PAGE Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.3.3 NADH + H+ + O2 Archaeoglobus fulgidus the enzyme may be involved in electron transfer reactions resulting in sulfate respiration NAD+ + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.3 Archaeoglobus fulgidus O29852
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.3
-
Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.3.3 1.3
-
pH 6.6, 55°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.3 additional information the enzyme shows diaphorase activity in the presence of electron acceptors such as tetrazolium and cytochrome c Archaeoglobus fulgidus ?
-
?
1.6.3.3 NADH + H+ + O2 the enzyme may be involved in electron transfer reactions resulting in sulfate respiration Archaeoglobus fulgidus NAD+ + H2O2
-
?
1.6.3.3 NADH + H+ + O2 no activity with beta-NADPH Archaeoglobus fulgidus NAD+ + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.6.3.3 ? x * 47000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
1.6.3.3 AF0395 assay at Archaeoglobus fulgidus
1.6.3.3 NoxA2
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.3.3 55
-
assay at Archaeoglobus fulgidus
1.6.3.3 80
-
-
Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.6.3.3 22 90 active from room temperature to 90°C. At 50°C, the activity of the recombinant enzyme is half that at 80°C Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.3.3 83
-
pH 7.6, 40 min, the half-life of the recombinant enzyme is 12 min, the partially purified native enzyme has a half-life of 35 h Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.3.3 6.6
-
assay at Archaeoglobus fulgidus
1.6.3.3 7.6
-
-
Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.3.3 4.4 8.4 pH 4.4: about 70% of maximal activity, pH 8.4: about 90% of maximal activity Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.3 FAD required for activity. 1.1 mol of FAD per mol of enzyme. The FAD cofactor is associated noncovalently with the protein Archaeoglobus fulgidus

General Information

EC Number General Information Comment Organism
1.6.3.3 physiological function the enzyme may be involved in electron transfer reactions resulting in sulfate respiration Archaeoglobus fulgidus