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Literature summary extracted from

  • Ma, K.; Adams, M.W.
    An unusual oxygen-sensitive, iron- and zinc-containing alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (1999), J. Bacteriol., 181, 1163-1170.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.071
-
 NADPH 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 0.071
-
 NADP+ 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 0.091
-
 phenylacetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 0.17
-
 acetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 0.2
-
 methylglyoxal 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 20
-
 NAD+ 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 29.4
-
 ethanol 80°C, pH not specified in the publication Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.2 cytoplasm
-
 Pyrococcus furiosus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.2 Fe2+ contains 1 gatom iron per subunit. The iron is present as a low-spin Fe(II) Pyrococcus furiosus
1.1.1.2 Zinc contains 1 gatom per subunit Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 44840
-
 6 * 44840, mass spectrometric analysis Pyrococcus furiosus
1.1.1.2 48000
-
 6 * 48000, SDS-PAGE Pyrococcus furiosus
1.1.1.2 270000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.2 acetaldehyde + NADPH + H+ Pyrococcus furiosus the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways ethanol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Pyrococcus furiosus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.2 oxygen sensitive, half-life in air is about 1 h at 23°C. It is stable under anaerobic conditions even at high temperature, with half-lives at 85 and 95°C of 160 and 7 h, respectively Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 72.3
-
 80°C, pH not specified in the publication Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1-butanol + NADP+ 169% of the activity compared to ethanol Pyrococcus furiosus butanal + NADPH + H+
-
 r
1.1.1.2 1-heptanol + NADP+ 86% of the activity compared to ethanol Pyrococcus furiosus heptanal + NADPH + H+
-
 r
1.1.1.2 1-hexanol + NADP+ 95% of the activity compared to ethanol Pyrococcus furiosus hexanal + NADPH + H+
-
 r
1.1.1.2 1-octanol + NADP+ 57% of the activity compared to ethanol Pyrococcus furiosus octanal + NADPH + H+
-
 r
1.1.1.2 1-pentanol + NADP+ 172% of the activity compared to ethanol Pyrococcus furiosus pentanal + NADPH + H+
-
 r
1.1.1.2 1-propanol + NADP+ 156% of the activity compared to ethanol Pyrococcus furiosus propanal + NADPH + H+
-
 r
1.1.1.2 2-phenylethanol + NADP+ 76% of the activity compared to ethanol Pyrococcus furiosus phenylacetaldehyde + NADPH + H+
-
 r
1.1.1.2 acetaldehyde + NADPH + H+
-
 Pyrococcus furiosus ethanol + NADP+
-
 r
1.1.1.2 acetaldehyde + NADPH + H+ the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways Pyrococcus furiosus ethanol + NADP+
-
 ?
1.1.1.2 ethanol + NAD+ kcat/Km for NAD+ is 1900fold lower compared to NADP+ Pyrococcus furiosus acetaldehyde + NADH + H+
-
 r
1.1.1.2 ethanol + NADP+ kcat/Km for NAD+ is 1900fold lower compared to NADP+ Pyrococcus furiosus acetaldehyde + NADPH + H+
-
 r
1.1.1.2 isobutanol + NADP+ 122% of the activity compared to ethanol Pyrococcus furiosus isobutanal + NADPH + H+
-
 r
1.1.1.2 methylglyoxal + NADPH 27% of the activity compared to ethanol Pyrococcus furiosus ?
-
 ?
1.1.1.2 additional information no activity with methanol, 2-propanol, glycerol, D-sorbitol Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.2 homohexamer 6 * 48000, SDS-PAGE Pyrococcus furiosus
1.1.1.2 homohexamer 6 * 44840, mass spectrometric analysis Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 90
-
 above Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 23
-
 anaerobic conditions, 24 h, stable Pyrococcus furiosus
1.1.1.2 85
-
 anaerobic conditions, half-life: 160 h Pyrococcus furiosus
1.1.1.2 95
-
 anaerobic conditions, half-life: 7 h Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.2 2.4
-
 NAD+ 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 3.7
-
 methylglyoxal 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 5.5
-
 acetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 6.3
-
 NADPH 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 10.4
-
 phenylacetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 15.8
-
 NADP+ 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 19.2
-
 ethanol 80°C, pH not specified in the publication Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 9.4 10.2 80°C, optimal pH for ethanol oxidation Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADP+ kcat/Km for NAD+ is 1900fold lower compared to NADP+ Pyrococcus furiosus
1.1.1.2 NADPH
-
 Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
1.1.1.2 physiological function the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.2 0.0028
-
 acetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 0.12
-
 NAD+ 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 0.65
-
 ethanol 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 18.5
-
 methylglyoxal 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 93
-
 NADPH 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 114
-
 phenylacetaldehyde 80°C, pH not specified in the publication Pyrococcus furiosus
1.1.1.2 223
-
 NADP+ 80°C, pH not specified in the publication Pyrococcus furiosus