BRENDA - Enzyme Database

A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2

Fuenmayor, S.L.; Wild, M.; Boyes, A.L.; Williams, P.A.; J. Bacteriol. 180, 2522-2530 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.172
expressed in Escherichia coli DH5alpha cells
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.172
18800
-
x * 48799 + x * 18800, calculated from amino acid sequence
Pseudomonas sp.
1.14.13.172
48799
-
x * 48799 + x * 18800, calculated from amino acid sequence
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.172
salicylate + NADH + H+ + O2
Pseudomonas sp.
-
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
?
1.14.13.172
salicylate + NADH + H+ + O2
Pseudomonas sp. U2
-
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
?
1.14.13.172
salicylate + NADPH + H+ + O2
Pseudomonas sp.
-
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
?
1.14.13.172
salicylate + NADPH + H+ + O2
Pseudomonas sp. U2
-
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.172
Pseudomonas sp.
O52379 and O52380
large oxygenase component and small oxagenase component
-
1.14.13.172
Pseudomonas sp. U2
O52379 and O52380
large oxygenase component and small oxagenase component
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.172
salicylate + NADH + H+ + O2
-
722496
Pseudomonas sp.
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
1.14.13.172
salicylate + NADH + H+ + O2
-
722496
Pseudomonas sp. U2
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
1.14.13.172
salicylate + NADPH + H+ + O2
-
722496
Pseudomonas sp.
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
1.14.13.172
salicylate + NADPH + H+ + O2
-
722496
Pseudomonas sp. U2
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.13.172
?
x * 48799 + x * 18800, calculated from amino acid sequence
Pseudomonas sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.172
NADH
-
Pseudomonas sp.
1.14.13.172
NADPH
-
Pseudomonas sp.
1.14.13.172
[2Fe-2S]-center
contains a Rieske-type iron-sulfur center
Pseudomonas sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.172
expressed in Escherichia coli DH5alpha cells
Pseudomonas sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.172
NADH
-
Pseudomonas sp.
1.14.13.172
NADPH
-
Pseudomonas sp.
1.14.13.172
[2Fe-2S]-center
contains a Rieske-type iron-sulfur center
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.172
18800
-
x * 48799 + x * 18800, calculated from amino acid sequence
Pseudomonas sp.
1.14.13.172
48799
-
x * 48799 + x * 18800, calculated from amino acid sequence
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.172
salicylate + NADH + H+ + O2
Pseudomonas sp.
-
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
?
1.14.13.172
salicylate + NADH + H+ + O2
Pseudomonas sp. U2
-
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
?
1.14.13.172
salicylate + NADPH + H+ + O2
Pseudomonas sp.
-
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
?
1.14.13.172
salicylate + NADPH + H+ + O2
Pseudomonas sp. U2
-
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.172
salicylate + NADH + H+ + O2
-
722496
Pseudomonas sp.
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
1.14.13.172
salicylate + NADH + H+ + O2
-
722496
Pseudomonas sp. U2
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
1.14.13.172
salicylate + NADPH + H+ + O2
-
722496
Pseudomonas sp.
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
1.14.13.172
salicylate + NADPH + H+ + O2
-
722496
Pseudomonas sp. U2
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.13.172
?
x * 48799 + x * 18800, calculated from amino acid sequence
Pseudomonas sp.