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Literature summary extracted from

  • Cheng, J.; Coyle, C.; Panaccione, D.; O'Connor, S.
    A role for old yellow enzyme in ergot alkaloid biosynthesis (2010), J. Am. Chem. Soc., 132, 1776-1777.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.100 expressed in Escherichia coli BL21(DE3) cells Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.100 0.0401
-
 NADPH pH and temperature not specified in the publication Aspergillus fumigatus
1.3.1.100 0.048
-
 chanoclavine-I aldehyde pH and temperature not specified in the publication Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.100 Aspergillus fumigatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.100 Ni-NTA column chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.100 chanoclavine-I aldehyde + NADPH + H+
-
 Aspergillus fumigatus dihydrochanoclavine-I aldehyde + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.100 EasA
-
 Aspergillus fumigatus
1.3.1.100 old yellow enzyme
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.100 38.5
-
 chanoclavine-I aldehyde pH and temperature not specified in the publication Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.100 NADPH
-
 Aspergillus fumigatus