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Literature summary extracted from

  • Janvary, L.; Hoffmann, T.; Pfeiffer, J.; Hausmann, L.; Töpfer, R.; Fischer, T.; Schwab, W.
    A double mutation in the anthocyanin 5-O-glucosyltransferase gene disrupts enzymatic activity in Vitis vinifera L. (2009), J. Agric. Food Chem., 57, 3512-3518.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.298 DNA and amino acid sequence determination and analysis of the enzyme from cultivar Regent, revealing five amino acid substitutions and a truncation at the C-terminus causing the inactivation of the enzyme Vitis vinifera

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.298 A259S naturally occuring mutation Vitis vinifera
2.4.1.298 I461M naturally occuring mutation Vitis vinifera
2.4.1.298 L110V naturally occuring mutation Vitis vinifera
2.4.1.298 L372 naturally occuring mutation Vitis vinifera
2.4.1.298 V121L for the naturally inactive enzyme from cultivar Regent, restoration of the C-terminus in the European allele alone proves to be insufficient for a reversal to a functional allele. An additional V121L transition located in close spatial vicinity of the catalytically active histidine in the active site of the nonfunctional protein is also essential to recover 5GT activity Vitis vinifera
2.4.1.298 V307L naturally occuring mutation Vitis vinifera

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.298 Vitis vinifera
-
-
-
2.4.1.298 Vitis vinifera Regent
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Vitis vinifera UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Vitis vinifera Regent UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
-
 Vitis vinifera UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
-
 Vitis vinifera Regent UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + malvidin 3-O-beta-D-glucoside
-
 Vitis vinifera UDP + malvidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + malvidin 3-O-beta-D-glucoside
-
 Vitis vinifera Regent UDP + malvidin 3,5-di-O-beta-D-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.298 More three-dimensional structure and modeling, overview Vitis vinifera

Synonyms

EC Number Synonyms Comment Organism
2.4.1.298 5GT
-
 Vitis vinifera
2.4.1.298 anthocyanin 5-O-glucosyltransferase
-
 Vitis vinifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.298 30
-
 assay at Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.298 7.5
-
 assay at Vitis vinifera

General Information

EC Number General Information Comment Organism
2.4.1.298 malfunction sequence analysis of the enzyme from cultivar Regent reveals five amino acid substitutions and a truncation at the C-terminus causing the inactivation of the enzyme Vitis vinifera