Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gautam, A.; Shukla, H.; Khan, S.; Rishi, P.; Bhakuni, V.; Tewari, R.
    Structural and functional features of UDP-N-acetylenolpyruvylglucosamine reductase of Mycobacterium tuberculosis H37Rv (2010), Int. J. Integr. Biol., 10, 66-75.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.98 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.98 Ca2+ complete inhibition at 10 mM Mycobacterium tuberculosis
1.3.1.98 guanidine hydrochloride an exponential decrease in enzymatic activity from 100% to 20% is observed between 0 and 0.05 M, no activity is observed above 0.5 M Mycobacterium tuberculosis
1.3.1.98 Urea no activity at 2.5 M Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.98 0.0425
-
NADPH pH and temperature not specified in the publication Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.98 K+ required for activation Mycobacterium tuberculosis
1.3.1.98 additional information not influenced by Mg2+ Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.98 40000
-
ESI mass spectrometry Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.98 Mycobacterium tuberculosis
-
-
-
1.3.1.98 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.98 Ni-NTA agarose column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.98 UDP-N-acetylenolpyruvylglucosamine + NADPH + H+
-
Mycobacterium tuberculosis UDP-N-acetylmuramate + NADP+
-
?
1.3.1.98 UDP-N-acetylenolpyruvylglucosamine + NADPH + H+
-
Mycobacterium tuberculosis H37Rv UDP-N-acetylmuramate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.3.1.98 monomer 1 * 40000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.3.1.98 EC 1.1.1.158 formerly Mycobacterium tuberculosis
1.3.1.98 MurB
-
Mycobacterium tuberculosis
1.3.1.98 UDP-N-acetylenolpyruvylglucosamine reductase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.98 30
-
-
Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.98 20 40 at 20 and 40°C more than 85% of the activity is retained Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.98 40 60 between 40°C and 60°C a fast decrease in enzyme activity is observed and above 60°C activity is completely lost Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.98 8
-
-
Mycobacterium tuberculosis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.98 8 10 any increase or decrease in pH from 8.0 results in significant drop in functional activity of the enzyme. Complete loss of activity occurs at pH 10.0 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.98 FAD
-
Mycobacterium tuberculosis