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Literature summary extracted from

  • Gao, Y.S.; Wang, Y.; Li, C.; Chen, Z.; Yan, Y.B.; Zhou, H.M.
    Dissecting the key residues crucial for the species-specific thermostability of muscle-type creatine kinase (2010), Int. J. Biol. Macromol., 47, 366-370.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.2 expressed in Escherichia coli Homo sapiens
2.7.3.2 expressed in Escherichia coli Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 A189D Km and kcat values similar to wild-type, T0.5 (°C): 46.2 (wild-type: 48.0°C) Danio rerio
2.7.3.2 A205S Km and kcat values similar to wild-type, T0.5 (°C): 46.6 (wild-type: 48.0°C) Danio rerio
2.7.3.2 A267H Km and kcat values similar to wild-type, T0.5 (°C): 56.9 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 A329S Km and kcat values similar to wild-type, T0.5 (°C): 56.6 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 C146N Km and kcat values similar to wild-type, T0.5 (°C): 52.4 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 D189A Km values 2-3fold decreased compared to wild-type, kcat 30% decreased compared to wild-type, T0.5 (°C): 57 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 E185Q Km (ATP) and (creatine) significantly higher compared to wild-type, kcat value 74% of wild-type, T0.5 (°C): 47.4 (wild-type: 48.0°C) Danio rerio
2.7.3.2 E46Q Km values 2-3fold decreased compared to wild-type, kcat 30% decreased compared to wild-type, T0.5 (°C): 50.1 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 H267A Km and kcat values similar to wild-type, T0.5 (°C): 47.6 (wild-type: 48.0°C) Danio rerio
2.7.3.2 K304T Km and kcat values similar to wild-type, T0.5 (°C): 57.1 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 K36L Km and kcat values similar to wild-type, T0.5 (°C): 46.7 (wild-type: 48.0°C) Danio rerio
2.7.3.2 L36K Km and kcat values similar to wild-type, T0.5 (°C): 47.3 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 N146C Km and kcat values similar to wild-type, T0.5 (°C): 51 (wild-type: 48.0°C) Danio rerio
2.7.3.2 Q185E Km values 2-3fold decreased compared to wild-type, kcat 30% decreased compared to wild-type, T0.5 (°C): 56.4 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 Q46E Km and kcat values similar to wild-type, T0.5 (°C): 50.9 (wild-type: 48.0°C) Danio rerio
2.7.3.2 S205A Km and kcat values similar to wild-type, T0.5 (°C): 58.1 (wild-type: 56.9°C) Homo sapiens
2.7.3.2 S329A Km and kcat values similar to wild-type, T0.5 (°C): 48.3 (wild-type: 48.0°C) Danio rerio
2.7.3.2 T304K Km and kcat values similar to wild-type, T0.5 (°C): 47.6 (wild-type: 48.0°C) Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.36
-
 ATP 25°C, pH not specified in the publication, mutant T304K Danio rerio
2.7.3.2 0.42
-
 ATP 25°C, pH not specified in the publication, mutant S329A Danio rerio
2.7.3.2 0.45
-
 ATP 25°C, pH not specified in the publication, mutant N146C Danio rerio
2.7.3.2 0.49
-
 ATP 25°C, pH not specified in the publication, wild-type Danio rerio
2.7.3.2 0.58
-
 ATP 25°C, pH not specified in the publication, mutant A205S Danio rerio
2.7.3.2 0.58
-
 ATP 25°C, pH not specified in the publication, mutant Q46E Danio rerio
2.7.3.2 0.62
-
 ATP 25°C, pH not specified in the publication, mutant H267A Danio rerio
2.7.3.2 0.62
-
 ATP 25°C, pH not specified in the publication, wild-type Homo sapiens
2.7.3.2 0.65
-
 ATP 25°C, pH not specified in the publication, mutant S205A Homo sapiens
2.7.3.2 0.73
-
 ATP 25°C, pH not specified in the publication, mutant A267H Homo sapiens
2.7.3.2 0.74
-
 ATP 25°C, pH not specified in the publication, mutant K304T Homo sapiens
2.7.3.2 0.74
-
 ATP 25°C, pH not specified in the publication, mutant L36K Homo sapiens
2.7.3.2 0.82
-
 ATP 25°C, pH not specified in the publication, mutant A189D Danio rerio
2.7.3.2 0.84
-
 ATP 25°C, pH not specified in the publication, mutant D189A Homo sapiens
2.7.3.2 0.85
-
 ATP 25°C, pH not specified in the publication, mutant K36L Danio rerio
2.7.3.2 0.91
-
 ATP 25°C, pH not specified in the publication, mutant C146N Homo sapiens
2.7.3.2 0.92
-
 ATP 25°C, pH not specified in the publication, mutant E185Q Danio rerio
2.7.3.2 1.09
-
 ATP 25°C, pH not specified in the publication, mutant E46Q Homo sapiens
2.7.3.2 1.24
-
 ATP 25°C, pH not specified in the publication, mutant Q185E Homo sapiens
2.7.3.2 1.38
-
 ATP 25°C, pH not specified in the publication, mutant A329S Homo sapiens
2.7.3.2 2 3.4 Creatine 25°C, pH not specified in the publication, mutant A329S Homo sapiens
2.7.3.2 5.9
-
 Creatine 25°C, pH not specified in the publication, wild-type Homo sapiens
2.7.3.2 6.6
-
 Creatine 25°C, pH not specified in the publication, mutant S205A Homo sapiens
2.7.3.2 7.1
-
 Creatine 25°C, pH not specified in the publication, mutant L36K Homo sapiens
2.7.3.2 8.7
-
 Creatine 25°C, pH not specified in the publication, mutant A267H Homo sapiens
2.7.3.2 8.9
-
 Creatine 25°C, pH not specified in the publication, mutant K304T Homo sapiens
2.7.3.2 10.5
-
 Creatine 25°C, pH not specified in the publication, mutant S329A Danio rerio
2.7.3.2 11
-
 Creatine 25°C, pH not specified in the publication, mutant N146C Danio rerio
2.7.3.2 11.1
-
 Creatine 25°C, pH not specified in the publication, mutant Q185E Homo sapiens
2.7.3.2 12.9
-
 Creatine 25°C, pH not specified in the publication, mutant C146N Homo sapiens
2.7.3.2 14.2
-
 Creatine 25°C, pH not specified in the publication, wild-type Danio rerio
2.7.3.2 14.8
-
 Creatine 25°C, pH not specified in the publication, mutant E46Q Homo sapiens
2.7.3.2 16.7
-
 Creatine 25°C, pH not specified in the publication, mutant H267A Danio rerio
2.7.3.2 17.1
-
 Creatine 25°C, pH not specified in the publication, mutant Q46E Danio rerio
2.7.3.2 17.8
-
 Creatine 25°C, pH not specified in the publication, mutant T304K Danio rerio
2.7.3.2 20.6
-
 Creatine 25°C, pH not specified in the publication, mutant D189A Homo sapiens
2.7.3.2 20.8
-
 Creatine 25°C, pH not specified in the publication, mutant A205S Danio rerio
2.7.3.2 21.6
-
 Creatine 25°C, pH not specified in the publication, mutant K36L Danio rerio
2.7.3.2 28.5
-
 Creatine 25°C, pH not specified in the publication, mutant A189D Danio rerio
2.7.3.2 44.1
-
 Creatine 25°C, pH not specified in the publication, mutant E185Q Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.2 ATP + creatine Homo sapiens
-
 ADP + creatine phosphate
-
 ?
2.7.3.2 ATP + creatine Danio rerio
-
 ADP + creatine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Danio rerio Q90X19
-
-
2.7.3.2 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Homo sapiens ADP + creatine phosphate
-
 ?
2.7.3.2 ATP + creatine
-
 Danio rerio ADP + creatine phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 hMMCK
-
 Homo sapiens
2.7.3.2 muscle-type creatine kinase
-
 Homo sapiens
2.7.3.2 muscle-type creatine kinase
-
 Danio rerio
2.7.3.2 zMMCK
-
 Danio rerio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.2 36
-
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.3.2 20 25 optimal temperature Danio rerio

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.3.2 48
-
 muscle-type creatine kinase of Danio rerio is less stable compared to human muscle-type creatine kinase, T0.5: 48°C Danio rerio
2.7.3.2 56.9
-
 muscle-type creatine kinase of Danio rerio is less stable compared to human muscle-type creatine kinase, T0.5: 56.9°C Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.2 106
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant Q185E Homo sapiens
2.7.3.2 112
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant A329S Homo sapiens
2.7.3.2 115
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant E46Q Homo sapiens
2.7.3.2 117
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant D189A Homo sapiens
2.7.3.2 119
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant E185Q Danio rerio
2.7.3.2 122
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant K304T Homo sapiens
2.7.3.2 126
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant K36L Danio rerio
2.7.3.2 128
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant T304K Danio rerio
2.7.3.2 132
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant Q46E Danio rerio
2.7.3.2 140
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant N146C Danio rerio
2.7.3.2 142
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant H267A Danio rerio
2.7.3.2 142
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant S329A Danio rerio
2.7.3.2 159
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, wild-type Danio rerio
2.7.3.2 163
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant A189D Danio rerio
2.7.3.2 170
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, wild-type Homo sapiens
2.7.3.2 173
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant A205S Danio rerio
2.7.3.2 173
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant C146N Homo sapiens
2.7.3.2 175
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant L36K Homo sapiens
2.7.3.2 178
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant S205A Homo sapiens
2.7.3.2 196
-
 Creatine co-substrate: ATP, 25°C, pH not specified in the publication, mutant A267H Homo sapiens