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Literature summary extracted from

  • Liu, J.Y.; Hurley, T.D.
    A new crystal form of mouse thiamin pyrophosphokinase (2011), Int. J. Biochem. Mol. Biol., 2, 111-118.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.2 expressed in Escherichia coli BL21 cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.6.2 complexed with thiamin diphosphate-Mg2+ or thiamin-Mg2+, hanging drop vapor diffusion method, using 1.15-1.3 M sodium citrate, 0.1 M Na-HEPES, pH 8.6, at 22°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.2 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.2 nickel-chelate chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.2 ATP + thiamine
-
 Mus musculus AMP + thiamine diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.2 homotetramer x-ray crystallography Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.6.2 thiamin pyrophosphokinase
-
 Mus musculus
2.7.6.2 TPK
-
 Mus musculus