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Literature summary extracted from

  • Koetzler, M.P.; Blank, S.; Behnken, H.N.; Alpers, D.; Bantleon, F.I.; Spillner, E.; Meyer, B.
    Formation of the immunogenic alpha1,3-fucose epitope: elucidation of substrate specificity and of enzyme mechanism of core fucosyltransferase A (2012), Insect Biochem. Mol. Biol., 42, 116-125.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.214 expressed in Spodoptera frugiperda Sf9 cells Apis mellifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.214 GDP strong product inhibition Apis mellifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.214 0.42
-
 GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc at 25°C, pH not specified in the publication Apis mellifera
2.4.1.214 1
-
 GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc at 25°C, pH not specified in the publication Apis mellifera

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.214 Apis mellifera
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.214 glycoprotein
-
 Apis mellifera

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.214 Ni-NTA column chromatography Apis mellifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.214 GDP-beta-L-fucose + (1->4)-beta-D-galactosyl-N-acetyl-D-glucosaminyl-L-Asn
-
 Apis mellifera GDP + (1->4)-beta-D-galactosyl-[alpha-(1->3)-L-fucosyl]-N-acetyl-D-glucosaminyl-L-Asn
-
 ?
2.4.1.214 GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
 Apis mellifera GDP + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)]GlcNAc
-
 ?
2.4.1.214 GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc
-
 Apis mellifera GDP + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.214 alpha1,3-fucosyltransferase
-
 Apis mellifera
2.4.1.214 fucosyltransferase A
-
 Apis mellifera
2.4.1.214 FucTA
-
 Apis mellifera

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.214 0.097
-
 GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc at 25°C, pH not specified in the publication Apis mellifera
2.4.1.214 0.11
-
 GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc at 25°C, pH not specified in the publication Apis mellifera