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Literature summary extracted from

  • Brockhausen, I.; Riley, J.G.; Joynt, M.; Yang, X.; Szarek, W.A.
    Acceptor substrate specificity of UDP-Gal:GlcNAc-R beta1,3-galactosyltransferase (WbbD) from Escherichia coli O7:K1 (2008), Glycoconj. J., 25, 663-673.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.303 medicine differences between mammalian and bacterial enzymes could be exploited in the design of specific glycosyltransferase inhibitors that block the synthesis of O-antigens Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.303 additional information requirement for divalent metal ion Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.303 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli the enzyme is involved O7 lipopolysaccharide biosynthesis UDP + beta-D-Gal-beta-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
?
2.4.1.303 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli VW187 (O7:K1) the enzyme is involved O7 lipopolysaccharide biosynthesis UDP + beta-D-Gal-beta-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.303 Escherichia coli Q03084
-
-
2.4.1.303 Escherichia coli VW187 (O7:K1) Q03084
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.303 additional information WbbD require at least one phosphate in the acceptor substrate which has to be directly linked to GlcNAc, and ise optimally active when GlcNAc is linked to diphosphate. No glycosyl transfer is observed with cytidine-monophosphate N-acetylneuraminic acid, UDP-GalNAc, GDP-Man or UDP-GlcNAc. WbbD does not recognize GlcNAc linked to a glycopeptide Escherichia coli ?
-
?
2.4.1.303 additional information WbbD require at least one phosphate in the acceptor substrate which has to be directly linked to GlcNAc, and ise optimally active when GlcNAc is linked to diphosphate. No glycosyl transfer is observed with cytidine-monophosphate N-acetylneuraminic acid, UDP-GalNAc, GDP-Man or UDP-GlcNAc. WbbD does not recognize GlcNAc linked to a glycopeptide Escherichia coli VW187 (O7:K1) ?
-
?
2.4.1.303 UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol. The enzyme does not require a specific length of the lipid chain but can act on GlcNAc-alpha-PO3-PO3-(CH2)R-OPh substrates where R can be 6, 11 or 16 carbons Escherichia coli UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
?
2.4.1.303 UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol. The enzyme does not require a specific length of the lipid chain but can act on GlcNAc-alpha-PO3-PO3-(CH2)R-OPh substrates where R can be 6, 11 or 16 carbons Escherichia coli VW187 (O7:K1) UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
?
2.4.1.303 UDP-alpha-D-galactose + 1-O-(16-phenoxyhexadecyl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol. The enzyme does not require a specific length of the lipid chain but can act on GlcNAc-alpha-PO3-PO3-(CH2)R-OPh substrates where R can be 6, 11 or 16 carbons Escherichia coli UDP + 1-O-(16-phenoxyhexadecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
?
2.4.1.303 UDP-alpha-D-galactose + 1-O-(16-phenoxyhexadecyl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol. The enzyme does not require a specific length of the lipid chain but can act on GlcNAc-alpha-PO3-PO3-(CH2)R-OPh substrates where R can be 6, 11 or 16 carbons Escherichia coli VW187 (O7:K1) UDP + 1-O-(16-phenoxyhexadecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
?
2.4.1.303 UDP-alpha-D-galactose + 1-O-(6-phenoxyhex-1-yl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol. The enzyme does not require a specific length of the lipid chain but can act on GlcNAc-alpha-PO3-PO3-(CH2)R-OPh substrates where R can be 6, 11 or 16 carbons Escherichia coli UDP + 1-O-(6-phenoxyhexadecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
?
2.4.1.303 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol the enzyme is involved O7 lipopolysaccharide biosynthesis Escherichia coli UDP + beta-D-Gal-beta-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
?
2.4.1.303 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol the enzyme is specific for UDP-galactose as donor substrate Escherichia coli UDP + beta-D-Gal-beta-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
?
2.4.1.303 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol the enzyme is involved O7 lipopolysaccharide biosynthesis Escherichia coli VW187 (O7:K1) UDP + beta-D-Gal-beta-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
?
2.4.1.303 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol the enzyme is specific for UDP-galactose as donor substrate Escherichia coli VW187 (O7:K1) UDP + beta-D-Gal-beta-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.303 UDP-Gal:GlcNAc-R beta1,3-galactosyltransferase
-
Escherichia coli
2.4.1.303 WbbD
-
Escherichia coli
2.4.1.303 WbbD beta3Gal-transferase
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.303 37
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.303 7
-
assay at Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.1.303 physiological function the enzyme is essential for O antigen synthesis in the VW187 strain Escherichia coli